Genome-wide identification of the Jatropha curcas MYB family and functional analysis of the abiotic stress responsive gene JcMYB2
文献类型：期刊论文
| 作者 | Peng, Xianjun; Liu, Hui; Wang, Dan; Shen, Shihua
|
| 刊名 | BMC GENOMICS
 |
| 出版日期 | 2016 |
| 卷号 | 17 |
| 关键词 | Abiotic and biotic stress MYB transcription factor Promoter Tolerance |
| ISSN号 | 1471-2164 |
| DOI | 10.1186/s12864-016-2576-7 |
| 文献子类 | Article |
| 英文摘要 | Background: The MYB family is one of the most abundant transcription factor families in plants. MYB proteins are involved in plant development, abiotic stress tolerance, hormone signal transduction and disease resistance. Here we perform genome-wide identification of MYB family transcription factors in an energy plant J. curcas, including determining family composition, phylogenetic evolution and functional prediction analysis. In addition, we further elucidate the function of the JcMYB2 gene. Methods: The phylogenetic trees were constructed by using the neighbor-joining method in MEGA 5.2. The biological functions of some JcMYBs were predicted according to orthology. The full length cDNA of JcMYB2 was cloned by using the RACE method. GUS histochemical staining was used to test the activity of the JcMYB2 promoter. Expression patterns of JcMYB2 were detected by using qPCR Transcriptional activity JcMYB2 were confirmed through yeast one hybrid. Subcellular Localization of JcMYB2 Protein were demonstrated by transient expression in the tobacco leaf. The function of JcMYB2 in salt and freezing tolerance were detected in transgenic plants. Results: A genome-wide analysis identified 128 MYB genes, including 123 R2R3-MYBs, 4 R1R2R3-MYBs and 1 4R-MYB. All of the R2R3-MYBs are further classified into 19 groups which indicated functional conservation among previously identified groups of R2R3-MYB proteins. Among of these newly identified MYBs, the JcMYB2 belongs to group G11 and its expression is induced obviously by cold, salt and MeJA (Methyl Jasmonate) and slightly by ABA (abscisic acid). JcMYB2 is localized to the nucleus and has transcriptional activity. JcMYB2 overexpressing plants are more tolerant to salt and cold stress than wild type plants. Tissue specific expression profiles showed that the JcMYB2 gene was expressed ubiquitously throughout the plant, with higher expression levels observed in the root. Conclusion: A comprehensive genome-wide analysis and phylogenetic relationship of R2R3-MYB subfamily in J. curcas present the global identification and functional prediction of JcR2R3-MYBs. Additionally, JcMYB2 regulates the stress response signaling networks by interacting with MeJA and ABA signaling pathway and functions in the root development of J. curcas. |
| 学科主题 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 出版地 | LONDON |
| WOS关键词 | TRANSCRIPTION FACTOR SUPERFAMILY ; EXPRESSION ANALYSIS ; SALT TOLERANCE ; COLD STRESS ; CELL-CYCLE ; ARABIDOPSIS ; PROTEINS ; DROUGHT ; RICE ; DOMAIN |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000372544000001 |
| 出版者 | BMC |
| 资助机构 | Knowledge Innovation Program through Chinese Academy of SciencesKnowledge Innovation Program of the Chinese Academy of Sciences [KZCX2-YW-359-2] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270653] ; Huimin Technology Demonstration Project of the National Modern Agricultural Science and Technology Achievements City [Z151100001015008] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25268]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Peng, Xianjun,Liu, Hui,Wang, Dan,et al. Genome-wide identification of the Jatropha curcas MYB family and functional analysis of the abiotic stress responsive gene JcMYB2[J]. BMC GENOMICS,2016,17. |
| APA | Peng, Xianjun,Liu, Hui,Wang, Dan,&Shen, Shihua.(2016).Genome-wide identification of the Jatropha curcas MYB family and functional analysis of the abiotic stress responsive gene JcMYB2.BMC GENOMICS,17. |
| MLA | Peng, Xianjun,et al."Genome-wide identification of the Jatropha curcas MYB family and functional analysis of the abiotic stress responsive gene JcMYB2".BMC GENOMICS 17(2016). |
入库方式： OAI收割
来源：植物研究所
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