Aquaporin8 regulates cellular development and reactive oxygen species production, a critical component of virulence in Botrytis cinerea
文献类型：期刊论文
| 作者 | An, Bang2; Li, Boqiang; Li, Hua2; Zhang, Zhanquan; Qin, Guozheng ; Tian, Shiping2
|
| 刊名 | NEW PHYTOLOGIST
 |
| 出版日期 | 2016 |
| 卷号 | 209期号:4页码:1668-1680 |
| 关键词 | aquaporin Botrytis cinerea cellular development reactive oxygen species (ROS) pathogenesis |
| ISSN号 | 0028-646X |
| DOI | 10.1111/nph.13721 |
| 文献子类 | Article |
| 英文摘要 | Aquaporins (AQPs) are ubiquitous in nearly all organisms, mediating selective and rapid flux of water across biological membranes. The role of AQPs in phytopathogenic fungi is poorly understood. Orthologs of AQP genes in Botrytis cinerea were identified and knocked out. The effects of AQPs on hyphal growth and conidiation, formation of infection structures and virulence on plant hosts were examined. The role of AQP8 in reactive oxygen species (ROS) production, distribution and transport were further determined. Among eight AQPs, only AQP8 was essential for the ability of B. cinerea to infect plants. AQP8 was demonstrated to be an intrinsic plasma membrane protein, which may function as a channel and mediate hydrogen peroxide uptake. Deletion of AQP8 in B. cinerea completely inhibited the development of conidia and infection structures, and significantly affected noxR expression. Further observations revealed that both AQP8 and noxR impacted ROS distribution in the hyphal tips of B. cinerea. Moreover, AQP8 affected the expression of a mitochondrial protein, NQO1. A knockout mutant of NQO1 was observed to display reduced virulence. These data lead to a better understanding of the important role of AQP8 in the development and pathogenesis of plant pathogens. |
| 学科主题 | Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1469-8137 |
| WOS关键词 | FUNCTIONAL-ANALYSIS ; NADPH OXIDASES ; HYDROGEN-PEROXIDE ; OXIDATIVE STRESS ; GENE FAMILY ; EXPRESSION ; PROTEINS ; PATHOGENICITY ; MYCORRHIZAL ; PLANTS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000373379800033 |
| 出版者 | WILEY |
| 资助机构 | National Basic Research Program of ChinaNational Basic Research Program of China [2013CB127103] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31530057, 31172004] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25269]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | An, Bang,Li, Boqiang,Li, Hua,et al. Aquaporin8 regulates cellular development and reactive oxygen species production, a critical component of virulence in Botrytis cinerea[J]. NEW PHYTOLOGIST,2016,209(4):1668-1680. |
| APA | An, Bang,Li, Boqiang,Li, Hua,Zhang, Zhanquan,Qin, Guozheng,&Tian, Shiping.(2016).Aquaporin8 regulates cellular development and reactive oxygen species production, a critical component of virulence in Botrytis cinerea.NEW PHYTOLOGIST,209(4),1668-1680. |
| MLA | An, Bang,et al."Aquaporin8 regulates cellular development and reactive oxygen species production, a critical component of virulence in Botrytis cinerea".NEW PHYTOLOGIST 209.4(2016):1668-1680. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。