中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 植物研究所
  3. 中国科学院植物研究所
  4. 中科院北方资源植物重点实验室
An aquaporin PvTIP4;1 from Pteris vittata may mediate arsenite uptake

文献类型：期刊论文

作者He, Zhenyan; Yan, Huili3; Chen, Yanshan3; Shen, Hongling3; Xu, Wenxiu; Zhang, Haiyan; Shi, Lei; Zhu, Yong-Guan; Ma, Mi
刊名NEW PHYTOLOGIST
出版日期2016
卷号209期号:2页码:746-761
关键词aromatic/arginine (Ar/R) arsenic (As) arsenite (As(III)) aquaporin Pteris vittata PvTIP4 1 uptake
ISSN号0028-646X
DOI10.1111/nph.13637
文献子类Article
英文摘要The fern Pteris vittata is an arsenic hyperaccumulator. The genes involved in arsenite (As (III)) transport are not yet clear. Here, we describe the isolation and characterization of a new P. vittata aquaporin gene, PvTIP4;1, which may mediate As(III) uptake. PvTIP4;1 was identified from yeast functional complement cDNA library of P. vittata. Arsenic toxicity and accumulating activities of PvTIP4;1 were analyzed in Saccharomyces cerevisiae and Arabidopsis. Subcellular localization of PvTIP4;1-GFP fusion protein in P. vittata protoplast and callus was conducted. The tissue expression of PvTIP4; 1 was investigated by quantitative real-time PCR. Site-directed mutagenesis of the PvTIP4;1 aromatic/arginine (Ar/R) domain was studied. Heterologous expression in yeast demonstrates that PvTIP4;1 was able to facilitate As(III) diffusion. Transgenic Arabidopsis showed that PvTIP4;1 increases arsenic accumulation and induces arsenic sensitivity. Images and FM4-64 staining suggest that PvTIP4;1 localizes to the plasma membrane in P. vittata cells. A tissue location study shows that PvTIP4;1 transcripts are mainly expressed in roots. Site-directed mutation in yeast further proved that the cysteine at the LE1 position of PvTIP4;1 Ar/R domain is a functional site. PvTIP4;1 is a new represented tonoplast intrinsic protein (TIP) aquaporin from P. vittata and the function and location results imply that PvTIP4;1 may be involved in As(III) uptake.
学科主题Plant Sciences
出版地HOBOKEN
电子版国际标准刊号1469-8137
WOS关键词MAJOR INTRINSIC PROTEINS ; ARSENATE REDUCTASE ; VACUOLAR MEMBRANE ; ARABIDOPSIS ; GLYCEROL ; FERN ; SELECTIVITY ; TRANSPORT ; FAMILY ; CHANNEL
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000373376500029
出版者WILEY
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370299, 31470549]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/25279]  
专题中科院北方资源植物重点实验室
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Xiamen 361021, Peoples R China
3.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714		 He, Zhenyan,Yan, Huili,Chen, Yanshan,et al. An aquaporin PvTIP4;1 from Pteris vittata may mediate arsenite uptake[J]. NEW PHYTOLOGIST,2016,209(2):746-761.
APA He, Zhenyan.,Yan, Huili.,Chen, Yanshan.,Shen, Hongling.,Xu, Wenxiu.,...&Ma, Mi.(2016).An aquaporin PvTIP4;1 from Pteris vittata may mediate arsenite uptake.NEW PHYTOLOGIST,209(2),746-761.
MLA He, Zhenyan,et al."An aquaporin PvTIP4;1 from Pteris vittata may mediate arsenite uptake".NEW PHYTOLOGIST 209.2(2016):746-761.

入库方式： OAI收割

来源：植物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。