中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 植物研究所
  3. 中国科学院植物研究所
  4. 中科院北方资源植物重点实验室
CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.)

文献类型：期刊论文

作者Ren, Chong1,4; Liu, Xianju1,4; Zhang, Zhan1,4; Wang, Yi1,4; Duan, Wei1; Li, Shaohua1; Liang, Zhenchang1,2
刊名SCIENTIFIC REPORTS
出版日期2016
卷号6
ISSN号2045-2322
DOI10.1038/srep32289
文献子类Article
英文摘要The type II clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 system (CRISPR/Cas9) has been successfully applied to edit target genes in multiple plant species. However, it remains unknown whether this system can be used for genome editing in grape. In this study, we described genome editing and targeted gene mutation in 'Chardonnay' suspension cells and plants via the CRISPR/Cas9 system. Two single guide RNAs (sgRNAs) were designed to target distinct sites of the L-idonate dehydrogenase gene (IdnDH). CEL I endonuclease assay and sequencing results revealed the expected indel mutations at the target site, and a mutation frequency of 100% was observed in the transgenic cell mass (CM) as well as corresponding regenerated plants with expression of sgRNA1/Cas9. The majority of the detected mutations in transgenic CM were 1-bp insertions, followed by 1-to 3-nucleotide deletions. Off-target activities were also evaluated by sequencing the potential off-target sites, and no obvious off-target events were detected. Our results demonstrated that the CRISPR/Cas9 system is an efficient and specific tool for precise genome editing in grape.
学科主题Multidisciplinary Sciences
出版地LONDON
WOS关键词CRISPR-CAS SYSTEM ; RNA-GUIDED ENDONUCLEASE ; GENETIC-TRANSFORMATION ; GENOME MODIFICATIONS ; TRANSGENIC PLANTS ; LATER GENERATIONS ; ANALYSIS REVEALS ; 1ST GENERATION ; NUCLEASES ; RICE
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000382239600001
出版者NATURE PUBLISHING GROUP
资助机构National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31572090] ; Agricultural Breeding Project of Ningxia Hui Autonomous Region [NXNYYZ20150203] ; Hundred Talents of Chinese Academy of Sciences
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/25284]  
专题中科院北方资源植物重点实验室
作者单位1.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Inst Bot, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Peoples R China
4.Chinese Acad Sci, Key Lab Plant Resource, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714		 Ren, Chong,Liu, Xianju,Zhang, Zhan,et al. CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.)[J]. SCIENTIFIC REPORTS,2016,6.
APA Ren, Chong.,Liu, Xianju.,Zhang, Zhan.,Wang, Yi.,Duan, Wei.,...&Liang, Zhenchang.(2016).CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.).SCIENTIFIC REPORTS,6.
MLA Ren, Chong,et al."CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.)".SCIENTIFIC REPORTS 6(2016).

入库方式： OAI收割

来源：植物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。