Compatibility of Diatom Valve Records With Sedimentary Ancient DNA Amplicon Data: A Case Study in a Brackish, Alkaline Tibetan Lake
文献类型：期刊论文
| 作者 | Anslan, Sten2,3; Kang, Wengang4; Dulias, Katharina4; Wuennemann, Bernd4,5; Echeverria-Galindo, Paula4; Boerner, Nicole4; Schwarz, Anja4; Liu, Yongqin6,7,8; Liu, Keshao6; Kuenzel, Sven1 |
| 刊名 | FRONTIERS IN EARTH SCIENCE
 |
| 出版日期 | 2022-03-07 |
| 卷号 | 10页码:14 |
| 关键词 | SedaDNA Tibetan Plateau Lake Nam Co metabarcoding microscopy sediment core |
| DOI | 10.3389/feart.2022.824656 |
| 英文摘要 | Lake sediments represent valuable and widely used archives for tracking environmental and biotic changes over time. Past aquatic communities are traditionally studied via morphological identification of the remains of organisms. However, molecular identification tools, such as DNA metabarcoding, have revolutionized the field of biomonitoring by enabling high-throughput and fast identification of organisms from environmental samples (e.g., sediments and soil). Sedimentary ancient DNA (sedaDNA) metabarcoding, an approach to track the biodiversity of target organisms from sediment cores, spanning thousands of years, has been successfully applied in many studies. However, researchers seldom explore how well the signals from sedaDNA data correlate with the fossil records of target organisms. This information is essential to infer past environmental conditions and community changes of bioindicators when the increasingly popular molecular identification method, metabarcoding, is desired instead of a morphological identification approach. In this study, we explore the correlations of diatom valve records across the last similar to 940 years with the diatom sedaDNA metabarcoding data from the same sediment core from lake Nam Co (Tibetan Plateau). Overall, the results from valve vs. sedaDNA data revealed concordant diatom richness as well as community patterns. However, several mismatches in the diatom taxonomic composition existed between the data sets. In general, sedaDNA data harbored much higher diatom diversity, but due to the lack of reference sequences in public databases, many molecular units (amplicon sequence variants) remained unclassified to lower taxonomic levels. As our study lake, Nam Co, is characterized by brackish water and alkaline pH, some likely cases for the observed taxonomic composition mismatches may be due to a valve dissolution issue. Nevertheless, significant drivers for the diatom richness and community structure largely corresponded between data sets. Both valve and sedaDNA data demonstrated similar breakpoints for historical diatom community shifts. A particularly strong shift in the diatom community structure occurred after similar to 1950 CE, which may be associated with abrupt environmental changes on the Tibetan Plateau. Altogether, our study indicates that environmentally driven signals reflected by the diatom communities are successfully recovered via microfossil as well as molecular identification methods. |
| WOS关键词 | NAM CO ; SEQUENCE ALIGNMENT ; SURFACE SEDIMENTS ; FRESH-WATER ; DISSOLUTION ; PRESERVATION ; PLATEAU ; QUANTIFICATION ; NANOFRUSTULUM ; DIVERSITY |
| 资助项目 | Deutsche Forschungsgemeinschaft (DFG) via the International Research Training Group "Geoecosystems in transition on the Tibetan Plateau (TransTiP)", DFG[317513741/GRK 2309] |
| WOS研究方向 | Geology |
| 语种 | 英语 |
| 出版者 | FRONTIERS MEDIA SA |
| WOS记录号 | WOS:000778369300001 |
| 资助机构 | Deutsche Forschungsgemeinschaft (DFG) via the International Research Training Group "Geoecosystems in transition on the Tibetan Plateau (TransTiP)", DFG ; Deutsche Forschungsgemeinschaft (DFG) via the International Research Training Group "Geoecosystems in transition on the Tibetan Plateau (TransTiP)", DFG ; Deutsche Forschungsgemeinschaft (DFG) via the International Research Training Group "Geoecosystems in transition on the Tibetan Plateau (TransTiP)", DFG ; Deutsche Forschungsgemeinschaft (DFG) via the International Research Training Group "Geoecosystems in transition on the Tibetan Plateau (TransTiP)", DFG ; Deutsche Forschungsgemeinschaft (DFG) via the International Research Training Group "Geoecosystems in transition on the Tibetan Plateau (TransTiP)", DFG ; Deutsche Forschungsgemeinschaft (DFG) via the International Research Training Group "Geoecosystems in transition on the Tibetan Plateau (TransTiP)", DFG ; Deutsche Forschungsgemeinschaft (DFG) via the International Research Training Group "Geoecosystems in transition on the Tibetan Plateau (TransTiP)", DFG ; Deutsche Forschungsgemeinschaft (DFG) via the International Research Training Group "Geoecosystems in transition on the Tibetan Plateau (TransTiP)", DFG |
| 源URL | [http://ir.iggcas.ac.cn/handle/132A11/105135]  |
| 专题 | 地质与地球物理研究所_中国科学院新生代地质与环境重点实验室 |
| 通讯作者 | Anslan, Sten; Kang, Wengang |
| 作者单位 | 1.Max Planck Inst Evolutionary Biol, Dept Evolutionary Genet, Plon, Germany 2.Univ Tartu, Inst Ecol & Earth Sci, Tartu, Estonia 3.Tech Univ Carolo Wilhelmina Braunschweig, Zool Inst, Braunschweig, Germany 4.Tech Univ Carolo Wilhelmina Braunschweig, Inst Geosyst & Bioindicat, Braunschweig, Germany 5.Southwest Jiaotong Univ, Fac Geosci & Environm Engn, Chengdu, Peoples R China 6.Chinese Acad Sci, Inst Tibetan Plateau Res, State Key Lab Tibetan Plateau Earth Syst, Resources & Environm TPESRE, Beijing, Peoples R China 7.Chinese Acad Sci, CAS Ctr Excellence Tibetan Plateau Earth Sci, Beijing, Peoples R China 8.Lanzhou Univ, Ctr Pan Third Pole Environm, Lanzhou, Peoples R China 9.Univ Tartu, Inst Technol, Tartu, Estonia 10.Chinese Acad Sci, Inst Geol & Geophys, Key Lab Cenozo Geol & Environm, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Anslan, Sten,Kang, Wengang,Dulias, Katharina,et al. Compatibility of Diatom Valve Records With Sedimentary Ancient DNA Amplicon Data: A Case Study in a Brackish, Alkaline Tibetan Lake[J]. FRONTIERS IN EARTH SCIENCE,2022,10:14. |
| APA | Anslan, Sten.,Kang, Wengang.,Dulias, Katharina.,Wuennemann, Bernd.,Echeverria-Galindo, Paula.,...&Schwalb, Antje.(2022).Compatibility of Diatom Valve Records With Sedimentary Ancient DNA Amplicon Data: A Case Study in a Brackish, Alkaline Tibetan Lake.FRONTIERS IN EARTH SCIENCE,10,14. |
| MLA | Anslan, Sten,et al."Compatibility of Diatom Valve Records With Sedimentary Ancient DNA Amplicon Data: A Case Study in a Brackish, Alkaline Tibetan Lake".FRONTIERS IN EARTH SCIENCE 10(2022):14. |
入库方式： OAI收割
来源：地质与地球物理研究所
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