Highly Sensitive and Specific Responses of Oyster Hemocytes to Copper Exposure: Single-Cell Transcriptomic Analysis of Different Cell Populations
文献类型：期刊论文
| 作者 | Meng, Jie1,2,5 ; Wang, Wen-Xiong3,4,5,6
|
| 刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
 |
| 出版日期 | 2022-02-15 |
| 卷号 | 56期号:4页码:2497-2510 |
| ISSN号 | 0013-936X |
| 关键词 | oyster Cu exposure scRNA-seq granulocytes heterogeneity responses |
| DOI | 10.1021/acs.est.1c07510 |
| 通讯作者 | Wang, Wen-Xiong(wx.wang@cityu.edu.hk) |
| 英文摘要 | Oyster hemocytes are the primary vehicles transporting and detoxifying metals and are regarded as important cells for the occurrence of colored oysters due to copper (Cu) contamination. However, its heterogeneous responses under Cu exposure have not been studied. Single-cell transcriptome profiling (scRNA-seq) provides high-resolution visual insights into tissue dynamics and environmental responses. Here, we used scRNA-seq to study the responses of different cell populations of hemocytes under Cu exposure in an estuarine oyster Crassostrea hongkongensis. The 1900 population-specific Cu-responsive genes were identified in 12 clusters of hemocytes, which provided a more sensitive technique for examining Cu exposure. The granulocyte, semi-granulocyte, and hyalinocyte had specific responses, while the granulocyte was the most important responsive cell type and displayed heterogeneity responses of its two subtypes. In one subtype, Cu was transported with metal transporters and chelated with Cu chaperons in the cytoplasm. Excess Cu disturbed oxidative phosphorylation and induced reactive oxygen species production. However, in the other subtype, endocytosis was mainly responsible for Cu internalization, which was sequestered in membrane-bound granules. Collectively, our results provided the first mRNA expression profile of hemocytes in oysters and revealed the heterogeneity responses under Cu exposure. |
| 资助项目 | National Natural Science Foundation of China[32102815] ; Youth Innovation Promotion Association of the Chinese Academy of Science[2019210] |
| WOS研究方向 | Engineering ; Environmental Sciences & Ecology |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000763099700040 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/178437]  |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Wang, Wen-Xiong |
| 作者单位 | 1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.City Univ Hong Kong, Res Ctr Oceans & Human Hlth, Shenzhen Res Inst, Shenzhen 518057, Peoples R China 4.City Univ Hong Kong, State Key Lab Marine Pollut, Hong Kong 999077, Peoples R China 5.Southern Marine Sci & Engn Guangdong Lab Guangzho, Guangzhou 511458, Peoples R China 6.City Univ Hong Kong, Sch Energy & Environm, Hong Kong 999077, Peoples R China |
| 推荐引用方式 GB/T 7714 | Meng, Jie,Wang, Wen-Xiong. Highly Sensitive and Specific Responses of Oyster Hemocytes to Copper Exposure: Single-Cell Transcriptomic Analysis of Different Cell Populations[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2022,56(4):2497-2510. |
| APA | Meng, Jie,&Wang, Wen-Xiong.(2022).Highly Sensitive and Specific Responses of Oyster Hemocytes to Copper Exposure: Single-Cell Transcriptomic Analysis of Different Cell Populations.ENVIRONMENTAL SCIENCE & TECHNOLOGY,56(4),2497-2510. |
| MLA | Meng, Jie,et al."Highly Sensitive and Specific Responses of Oyster Hemocytes to Copper Exposure: Single-Cell Transcriptomic Analysis of Different Cell Populations".ENVIRONMENTAL SCIENCE & TECHNOLOGY 56.4(2022):2497-2510. |
入库方式： OAI收割
来源：海洋研究所
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