UGT440A1 Is Associated With Motility, Reproduction, and Pathogenicity of the Plant-Parasitic Nematode Bursaphelenchus xylophilus
文献类型：期刊论文
| 作者 | Wang, Min3; Du, Guicai4; Fang, Junna1; Wang, Linsong2; Guo, Qunqun4; Zhang, Tingting4; Li, Ronggui4 |
| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2022-05-31 |
| 卷号 | 13页码:15 |
| 关键词 | Bursaphelenchus xylophilus UDP-glycosyltransferase motility reproduction pathogenicity |
| ISSN号 | 1664-462X |
| DOI | 10.3389/fpls.2022.862594 |
| 通讯作者 | Li, Ronggui(lrg@qdu.edu.cn) |
| 英文摘要 | Pine wilt disease (PWD) caused by Bursaphelenchus xylophilus is considered a major threat to pine forests worldwide. Uridine diphosphate (UDP)-glycosyltransferases (UGTs) catalyze the conjugation of small lipophilic compounds with sugars and play crucial roles in the detoxification and homeostatic processes in all living organisms. We investigated the molecular characteristics and biological functions of the gene UGT440A1 that encodes UGTs in B. xylophilus. The in situ hybridization results indicated that UGT440A1 is expressed in all developmental stages of B. xylophilus, particularly in the head, intestine, and hypodermis of the second-stage of juveniles (J2), third-stage of juveniles (J3) and fourth-stage of juveniles (J4) females and in almost the whole body of J4 males and adults. Recombinant UGT440A1 was observed mainly in the inclusion bodies, and the enzyme activity assay revealed that UGT440A1 could catalyze the glycosylation reaction of two types of flavonols (kaempferol and quercetin). RNA interference (RNAi) of UGT440A1 suppressed motility, feeding, and reproduction of B. xylophilus. Furthermore, UGT440A1 knockdown caused a delay in the development of PWD symptoms in the pine seedlings inoculated with the nematodes. These results suggest that UGT440A1 is involved in the pathogenic process of B. xylophilus and the information may facilitate a better understanding of the molecular mechanism of PWD. |
| 资助项目 | Key Programs for Research and Development of Shandong Province, China[2016GNC110024] ; National Natural Science Foundation of China[31070575] ; National Natural Science Foundation of China[31901313] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000810931300001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/179533]  |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Li, Ronggui |
| 作者单位 | 1.Qingdao JiMo Peoples Hosp, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 3.Qingdao Univ, Med Coll, Qingdao, Peoples R China 4.Qingdao Univ, Coll Life Sci, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Min,Du, Guicai,Fang, Junna,et al. UGT440A1 Is Associated With Motility, Reproduction, and Pathogenicity of the Plant-Parasitic Nematode Bursaphelenchus xylophilus[J]. FRONTIERS IN PLANT SCIENCE,2022,13:15. |
| APA | Wang, Min.,Du, Guicai.,Fang, Junna.,Wang, Linsong.,Guo, Qunqun.,...&Li, Ronggui.(2022).UGT440A1 Is Associated With Motility, Reproduction, and Pathogenicity of the Plant-Parasitic Nematode Bursaphelenchus xylophilus.FRONTIERS IN PLANT SCIENCE,13,15. |
| MLA | Wang, Min,et al."UGT440A1 Is Associated With Motility, Reproduction, and Pathogenicity of the Plant-Parasitic Nematode Bursaphelenchus xylophilus".FRONTIERS IN PLANT SCIENCE 13(2022):15. |
入库方式： OAI收割
来源：海洋研究所
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