Characterization of a type III polyketide synthase (sjPKS1) gene and its expression in response to light in the economic brown alga Saccharina japonica
文献类型：期刊论文
| 作者 | Li, Jing1,2,3 ; Pang, Shaojun1,2,3; Su, Li1,2,3; Shan, Tifeng1,2,3
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| 刊名 | JOURNAL OF APPLIED PHYCOLOGY
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| 出版日期 | 2022-04-21 |
| 页码 | 11 |
| 关键词 | Kelp Type III polyketide synthase Phlorotannins Yeast one-hybrid library Transcription factor |
| ISSN号 | 0921-8971 |
| DOI | 10.1007/s10811-022-02736-9 |
| 通讯作者 | Pang, Shaojun(sjpang@ms.qdio.ac.cn) |
| 英文摘要 | Phlorotannins are among the most important defensive molecules in kelp. They have been majorly identified in brown algae so far. Type III polyketide synthase (PKS) is regarded as one of the enzymes catalyzing a major step in the biosynthetic pathway of phlorotannins. In this study, a type III PKS gene (sjPKS1) is characterized from the economic brown alga Saccharina japonica sporophyte. It contains 7 exons and 6 introns. The sjPKS1 open reading frame consists of 1245 nucleotides encoding a protein of 414 amino acids. The sjPKS1 are 87%, 83%, and 82% identical to that of Ectocarpus siliculosus, Sargassum binderi, and Sargassum fusiforme at the nucleotide level, respectively. While the amino acid sequences of sjPKS1 share more than 90% identity to that of the three brown algae species. The sjPKS1 expression at 200 mu mol photons m(-2) s(-1) was significantly higher than at 20 and 50 mu mol photons m(-2) s(-1) in sporophytic seedlings, so did the content of soluble phlorotannins inside the plants. Seven motifs in response to light are identified in the 5 ' flanking region of sjPKS1 and two potential transcription factors are predicted by screening yeast one-hybrid library. |
| 资助项目 | National Natural Science Foundation of China[31702365] ; Biological Resources Program from Chinese Academy of Sciences[KFJ-BRP-017-53] ; STS program from the Chinese Academy of Sciences[KFJ-STS-QYZD-154] ; Chinese Academy of Science[COMS2020J01] ; Chinese Academy of Science[CZBZX1] ; China Agriculture Research System[CARS-50] ; Taishan Scholar Program of Shandong Province ; National Marine Genetic Resource Center |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000784616500001 |
| 出版者 | SPRINGER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/178744]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Pang, Shaojun |
| 作者单位 | 1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jing,Pang, Shaojun,Su, Li,et al. Characterization of a type III polyketide synthase (sjPKS1) gene and its expression in response to light in the economic brown alga Saccharina japonica[J]. JOURNAL OF APPLIED PHYCOLOGY,2022:11. |
| APA | Li, Jing,Pang, Shaojun,Su, Li,&Shan, Tifeng.(2022).Characterization of a type III polyketide synthase (sjPKS1) gene and its expression in response to light in the economic brown alga Saccharina japonica.JOURNAL OF APPLIED PHYCOLOGY,11. |
| MLA | Li, Jing,et al."Characterization of a type III polyketide synthase (sjPKS1) gene and its expression in response to light in the economic brown alga Saccharina japonica".JOURNAL OF APPLIED PHYCOLOGY (2022):11. |
入库方式： OAI收割
来源：海洋研究所
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