Vaccine Adjuvants Induce Formation of Intraperitoneal Extracellular Traps in Flounder (Paralichthys olivaceus)
文献类型：期刊论文
| 作者 | Li, Qian4 ; Chi, Heng3,4; Shi, Xueyan4; Gan, Qiujie4; Dalmo, Roy Ambli2; Sun, Yuan-yuan1; Tang, Xiaoqian3,4; Xing, Jing3,4; Sheng, Xiuzhen3,4; Zhan, Wenbin3,4
|
| 刊名 | FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
 |
| 出版日期 | 2022-03-30 |
| 卷号 | 12页码:11 |
| ISSN号 | 2235-2988 |
| 关键词 | extracellular traps (ETs) antigen adjuvant vaccination fish |
| DOI | 10.3389/fcimb.2022.875409 |
| 通讯作者 | Chi, Heng(chiheng_2001@163.com) |
| 英文摘要 | Adjuvants are used to increase the strength, quality, and duration of the immune response of vaccines. Neutrophils are the first immune cells that arrive at the injection site and can release DNA fibers together with granular proteins, so-called neutrophil extracellular traps (NETs), to entrap microbes in a sticky matrix of extracellular chromatin and microbicidal agents. Similar extracellular structures were also released by macrophages, mast cells, and eosinophils and are now generalized as "ETs." Here we demonstrated that Alum adjuvant stimulation led to peritoneal cells swarming and ET release in vitro. Moreover, compared to antigen stimulation alone, ET release was significantly increased after stimulation with antigen-mixed adjuvants and in a time- and dose-dependent manner. In vivo, we were able to monitor and quantify the continuous changes of the ET release in the same fish by using the small animal in vivo imaging instrument at different times during the early stages after intraperitoneal immunization. The results showed that the fluorescence signal of ETs in the peritoneum increased from 0 to 12 h after injection and then gradually decreased. The fluorescence signals came from extracellular DNA fibers, which are sensitive to DNase I and confirmed by microscopy of peritoneal fluid ex vivo. In summary, this study introduced a new method for detecting ETs in the peritoneum of fish in vivo and indicated that ET formation is involved in the immune response at the early stage after intraperitoneal immunization to vaccines. |
| 资助项目 | National Natural Science Foundation of China[31872594] ; National Natural Science Foundation of China[31730101] ; National Key Research and Development Program of China[2019YFD0900101] ; National Key Research and Development Program of China[2019YFD0900102] |
| WOS研究方向 | Immunology ; Microbiology |
| 语种 | 英语 |
| 出版者 | FRONTIERS MEDIA SA |
| WOS记录号 | WOS:000784019200001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/178777]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Chi, Heng |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Univ Tromso, Arctic Univ Norway, Norwegian Coll Fishery Sci, Fac Biosci Fisheries & Econ, Tromso, Norway 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China 4.Ocean Univ China, Lab Pathol & Immunol Aquat Anim, KLMME, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Qian,Chi, Heng,Shi, Xueyan,et al. Vaccine Adjuvants Induce Formation of Intraperitoneal Extracellular Traps in Flounder (Paralichthys olivaceus)[J]. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,2022,12:11. |
| APA | Li, Qian.,Chi, Heng.,Shi, Xueyan.,Gan, Qiujie.,Dalmo, Roy Ambli.,...&Zhan, Wenbin.(2022).Vaccine Adjuvants Induce Formation of Intraperitoneal Extracellular Traps in Flounder (Paralichthys olivaceus).FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,12,11. |
| MLA | Li, Qian,et al."Vaccine Adjuvants Induce Formation of Intraperitoneal Extracellular Traps in Flounder (Paralichthys olivaceus)".FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 12(2022):11. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。