Ubiquitin-proteasome pathway plays an essential regulatory role during spermatangium formation in Neopyropia yezoensis
文献类型：期刊论文
| 作者 | Niu, Jianfeng2,3,4; Yang, Jiali2,5 ; Feng, Jianhua2; Feng, Zezhong1,2; Wang, Xia2,5; Yu, Bin2,5; Wang, Guangce2,3,4
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| 刊名 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
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| 出版日期 | 2022-03-01 |
| 卷号 | 62页码:12 |
| ISSN号 | 2211-9264 |
| 关键词 | Neopyropia yezoensis Ubiquitin 26S proteasome Sexual differentiation Spermatangium Molecular mechanism |
| DOI | 10.1016/j.algal.2021.102623 |
| 通讯作者 | Wang, Guangce(gcwang@qdio.ac.cn) |
| 英文摘要 | Gerproductive cell formation is one of the most important physiological processes of organisms. The Rhodophyta, Neopyropia yezoensis, is a typical Archaeplastida and the spermatangia is usually formed along the edge of thalli and visible to the naked eye. It is therefore meaningful to invest the mechanisms of cell development. Here, the cell clumps at four typical differentiation stages of spermatangium in N. yezoensis were collected and transcriptome analysis was performed. A total of 12,188 differentially expressed genes (DEGs) were identified and the number of DEGs increased compared to the control as spermatangium formation progressed. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that carbon fixation, glycolysis/gluconeogenesis, photosynthesis-antenna proteins and some primary metabolic processes were down-regulated. In contrast, sphingolipid metabolism, inositol phosphate metabolism, ubiquitin mediated proteolysis and DNA replication were up-regulated. The development of spermatangia is believed to be induced by the sex signaling molecules due to the up-regulation of sphingolipid metabolism, the biosynthesis of phytosterol and the expression of a steroid bound receptor of heme-binding b5-domain containing protein at the initial stage of spermatangia formation. During the course of development, a total of 163 DEGs related to the ubiquitin-proteasome (Ub-proteasome) system were identified and half of these were up-regulated. Additionally, a series of ubiquitin-protein ligases (E3s) related to development, cell cycle, and chromosome replication were analyzed by qRT-PCR. The continuously upregulated of histone H2A/H2B implied that the shift of global gene expression profile modulated by the Ub-proteasome system was necessary during the differentiation. The up-regulation of the anaphasepromoting complex (APC) and its related protein factors meant that ubiquitin-mediated proteolysis enhanced the cell-cycle and chromosome replication. All the results suggest that the development of male germ cells is a highly modulated process and the Ub-proteasome system might play an essential regulatory role. |
| 资助项目 | National Natural Science Foundation of China[41776150] ; National Natural Science Foundation of China[41476140] ; China Agriculture Research System of MOF ; Key Deployment Project of the Centre for OceanMega-Research of Science ; Chinese Academy of Sciences ; Major Scientific and Technological Innova-tion Project of Shandong Provincial Key Research and Development Program ; [COMS2019Q02] ; [2019JZZY010815] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000787890400009 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/178873]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Guangce |
| 作者单位 | 1.Qingdao Agr Univ, Marine Sci & Engn Coll, Qingdao 266109, Peoples R China 2.Chinese Acad Sci IOCAS, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China 4.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, 168 Wenhai Middle Rd, Qingdao 266237, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Niu, Jianfeng,Yang, Jiali,Feng, Jianhua,et al. Ubiquitin-proteasome pathway plays an essential regulatory role during spermatangium formation in Neopyropia yezoensis[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2022,62:12. |
| APA | Niu, Jianfeng.,Yang, Jiali.,Feng, Jianhua.,Feng, Zezhong.,Wang, Xia.,...&Wang, Guangce.(2022).Ubiquitin-proteasome pathway plays an essential regulatory role during spermatangium formation in Neopyropia yezoensis.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,62,12. |
| MLA | Niu, Jianfeng,et al."Ubiquitin-proteasome pathway plays an essential regulatory role during spermatangium formation in Neopyropia yezoensis".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 62(2022):12. |
入库方式： OAI收割
来源：海洋研究所
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