A Type Ib Crustin from Deep-Sea Shrimp Possesses Antimicrobial and Immunomodulatory Activity
文献类型：期刊论文
| 作者 | Wang, Yu-Jian1,2,3 ; Sun, Li1,2,3
|
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2022-06-01 |
| 卷号 | 23期号:12页码:14 |
| 关键词 | crustin WAP domain bactericidal deep-sea |
| DOI | 10.3390/ijms23126444 |
| 通讯作者 | Sun, Li(lsun@qdio.ac.cn) |
| 英文摘要 | Crustins are small antimicrobial proteins produced by crustaceans. Of the many reported crustins, very few are from deep sea environments. Crustins are categorized into several types. Recently, the Type I crustin has been further classified into three subtypes, one of which is Type Ib, whose function is unknown. Here, we studied the function of a Type Ib crustin (designated Crus2) identified from a deep-sea crustacean. Crus2 has a whey acidic protein (WAP) domain and a long C-terminal region (named P58). Recombinant Crus2 bound to peptidoglycan (PGN), lipoteichoic acid (LTA), and lipopolysaccharide (LPS), and killed Gram-positive and Gram-negative bacteria by permeabilizing the bacterial cytomembrane. Consistently, Crus2 dramatically attenuated the inflammatory response induced by LPS and LTA. Disruption of the disulfide bonds in the WAP domain abolished the bactericidal ability of Crus2, but had no effect on the bacterial binding ability of Crus2. Deletion of the C-terminal P58 region moderately affected the antimicrobial activity of Crus2 against some bacteria. P58 as a synthesized peptide could bind bacteria and inhibit the bactericidal activity of Crus2. Taken together, these results revealed different roles played by the WAP domain and the P58 region in Type Ib crustin, and provided new insights into the antimicrobial and immunomodulatory functions of crustins. |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050403] ; Taishan Scholar Program of Shandong Province |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000816377900001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/179139]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Sun, Li |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yu-Jian,Sun, Li. A Type Ib Crustin from Deep-Sea Shrimp Possesses Antimicrobial and Immunomodulatory Activity[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2022,23(12):14. |
| APA | Wang, Yu-Jian,&Sun, Li.(2022).A Type Ib Crustin from Deep-Sea Shrimp Possesses Antimicrobial and Immunomodulatory Activity.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(12),14. |
| MLA | Wang, Yu-Jian,et al."A Type Ib Crustin from Deep-Sea Shrimp Possesses Antimicrobial and Immunomodulatory Activity".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.12(2022):14. |
入库方式： OAI收割
来源：海洋研究所
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