Overexpression of OHPs in Neopyropia yezoensis (Rhodophyta) reveals their possible physiological roles & nbsp;
文献类型：期刊论文
| 作者 | Zheng, Zhenbing1; He, Bangxiang1,2; Guo, Meng Lin1; Xie, Xiujun1; Huan, Li1 ; Zhang, Baoyu1; Shao, Zhizhuo1; Wang, Guangce1,3
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| 刊名 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
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| 出版日期 | 2022-05-01 |
| 卷号 | 64页码:10 |
| ISSN号 | 2211-9264 |
| 关键词 | One helix proteins Overexpression NPQ Neopyropia yezoensis Transgene |
| DOI | 10.1016/j.algal.2022.102714 |
| 通讯作者 | Wang, Guangce(gcwang@qdio.ac.cn) |
| 英文摘要 | The red macroalga Neopyropia yezoensis is a model organism, and an economically important seaweed widely cultured in eastern Asian countries. Transgenic research on N. yezoensis is difficult and limited to reporter genes and selection markers due to the specificities of this species. In this study, plasmids were constructed to over express six one helix protein (OHP) coding genes in N. yezoensis, and four transgenic lines with high expression levels of the target genes were obtained. The photosynthetic parameters were determined and it was found that the overexpression of OHPs altered electron transport in photosystem I and photosystem II, which was indicated by the increased 1-qL values and decreased P700(+) reduction rates. The results also showed that the non photochemical quenching (NPQ) kinetics in N. yezoensis could not be stimulated by high light (HL) treatment. Two of the four transgenic lines showed stimulated NPQ, and they did not exhibit an obvious increased ability to resist to HL, which was shown by the decreased Fv/Fm values. The increased H2O2 content and decreased peroxidase (POD) activity were considered the possible results of the overexpressed OHPs, and they were responsible for the enhanced NPQ and HL sensitivity in those two transgenic lines.& nbsp;& nbsp; |
| 资助项目 | National Key Research and Development Program of China[2018YFD0901500] ; National Natural Science Foundation of China[41906105] ; National Natural Science Foundation of China[41606161] ; National Natural Science Foundation of China[41976097] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000794241000013 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/179266]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Guangce |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Anhui Polytech Univ, Coll Biol & Food Engn, Wuhu, Anhui, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 |
Zheng, Zhenbing,He, Bangxiang,Guo, Meng Lin,et al. Overexpression of OHPs in Neopyropia yezoensis (Rhodophyta) reveals their possible physiological roles & nbsp; [J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2022,64:10. |
| APA |
Zheng, Zhenbing.,He, Bangxiang.,Guo, Meng Lin.,Xie, Xiujun.,Huan, Li.,...&Wang, Guangce.(2022). Overexpression of OHPs in Neopyropia yezoensis (Rhodophyta) reveals their possible physiological roles & nbsp; .ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,64,10. |
| MLA |
Zheng, Zhenbing,et al." Overexpression of OHPs in Neopyropia yezoensis (Rhodophyta) reveals their possible physiological roles & nbsp; ".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 64(2022):10. |
入库方式： OAI收割
来源：海洋研究所
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