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The mechanism by which Enteromorpha Linza polysaccharide promotes Bacillus subtilis growth and nitrate removal

文献类型：期刊论文

作者Zhang, Hui1,2,3,5; Chen, Xiaolin1,3,4; Song, Lin5; Liu, Song1,3,4; Li, Pengcheng1,3,4
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
出版日期2022-06-01
卷号209页码:840-849
关键词Enteromorpha Linza polysaccharide Bacillus subtilis Growth promotion Nitrate removal Mechanism
ISSN号0141-8130
DOI10.1016/j.ijbiomac.2022.04.082
通讯作者Chen, Xiaolin(chenxl@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn)
英文摘要In this study, we discussed the relationship between Entermorpha linza polysaccharide (EP) and Bacillus subtilis, which can transform nitrate. A sole carbon source experiment showed that Bacillus subtilis could utilize EP, and the bacterial density was maximally increased by 54.43% in the EP groups. The results of reducing sugar determination proved the secretion of polysaccharide-degrading enzymes. Scanning electron microscopy (SEM) showed that the EP groups had fewer spores and shrunken bacteria, indicating that EP could improve the growth environment and maintain bacterial integrity. Additionally, the ratios of periplasmic nitrate reductase (NAP), nitrite reductase (NIR), and dissimilatory nitrate reductase (D-NRase) in the EP groups were maximally increased by 107.22%, 84.70% and 36.10%, respectively. Transcriptome analysis further confirmed the above mentioned results. For example, the high expression of quorum sensing genes indicated that EP groups had higher bacterial density. Moreover, the high expression of antioxidant genes in the EP groups may be related to morphological integrity. Our study provides a basis for further discussion of the mechanism.
资助项目National Key R&D Program of China[2018YFD0901104]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种英语
WOS记录号WOS:000796998300001
出版者ELSEVIER
源URL[http://ir.qdio.ac.cn/handle/337002/179344]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Chen, Xiaolin; Li, Pengcheng
作者单位1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Qingdao 266071, Peoples R China
4.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China
5.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Qingdao 266042, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Hui,Chen, Xiaolin,Song, Lin,et al. The mechanism by which Enteromorpha Linza polysaccharide promotes Bacillus subtilis growth and nitrate removal[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2022,209:840-849.
APA Zhang, Hui,Chen, Xiaolin,Song, Lin,Liu, Song,&Li, Pengcheng.(2022).The mechanism by which Enteromorpha Linza polysaccharide promotes Bacillus subtilis growth and nitrate removal.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,209,840-849.
MLA Zhang, Hui,et al."The mechanism by which Enteromorpha Linza polysaccharide promotes Bacillus subtilis growth and nitrate removal".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 209(2022):840-849.

入库方式： OAI收割

来源：海洋研究所

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