Use of quantitative real-time PCR to select reference genes in the testis and ovary of Apostichopus japonicus during the breeding period
文献类型：期刊论文
| 作者 | Zhang, Shuangyan1,2,3,4,5,6; Ru, Xiaoshang1,2,3,4,6; Su, Fang1,2,3,4,5,6 ; Liang, Wenke1,2,3,4,6; Zhang, Libin1,2,3,4,5,6; Yang, Hongsheng1,2,3,4,5,6,7
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| 刊名 | AQUACULTURE REPORTS
 |
| 出版日期 | 2022-02-01 |
| 卷号 | 22页码:7 |
| 关键词 | Expression stability Gonads Quantitative real-time PCR Reference genes Sea cucumber breeding period |
| ISSN号 | 2352-5134 |
| DOI | 10.1016/j.aqrep.2022.101010 |
| 通讯作者 | Zhang, Libin(zhanglibin@qdio.ac.cn) |
| 英文摘要 | Gonads play a critical role in the reproduction of sea cucumbers, and their development is regulated by a large number of genes. To date, the ideal reference genes (RGs) for studying sea cucumber gonadal development have not been reported. In this study, we selected ten RGs and conducted quantitative real-time PCR analysis to identify the most stable among Apostichopus japonicus gonads at four different stages of gonadal development. We used delta C-q, GeNorm, NormFinder, BestKeeper, and RefFinder to evaluate the stability of candidate genes. EF1 beta and Grb2 together were the preferred combination of stable RGs among all of the gonad samples and for the ovaries alone. UBQ combined with RPS4 was the best combination of stable RGs in the testes. This study aimed to provide a basis for analysis of differences in gene expression related to gonadal development in sea cucumbers, and the results provide valuable information for further studies of the molecular mechanisms involved in sea cucumber gonadal development. |
| 资助项目 | National Natural Science Foundation of China[42061160365] ; National Natural Science Foundation of China[41876157] ; Scientific Research and Tech-nological Development Program of Fangchenggang[AB20014021] ; International Partners Program of the Chinese Academy of Sciences[133137KYSB20180069] |
| WOS研究方向 | Fisheries |
| 语种 | 英语 |
| WOS记录号 | WOS:000780442200001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/178741]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Zhang, Libin |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 7.Chinese Acad Sci, Innovat Seed Design, Wuhan 430000, Peoples R China |
| 推荐引用方式 GB/T 7714 |
Zhang, Shuangyan,Ru, Xiaoshang,Su, Fang,et al. Use of quantitative real-time PCR to select reference genes in the testis and ovary of Apostichopus japonicus during the breeding period [J]. AQUACULTURE REPORTS,2022,22:7. |
| APA |
Zhang, Shuangyan,Ru, Xiaoshang,Su, Fang,Liang, Wenke,Zhang, Libin,&Yang, Hongsheng.(2022). Use of quantitative real-time PCR to select reference genes in the testis and ovary of Apostichopus japonicus during the breeding period .AQUACULTURE REPORTS,22,7. |
| MLA |
Zhang, Shuangyan,et al." Use of quantitative real-time PCR to select reference genes in the testis and ovary of Apostichopus japonicus during the breeding period ".AQUACULTURE REPORTS 22(2022):7. |
入库方式： OAI收割
来源：海洋研究所
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