Gonad Transcriptome and Whole-Genome DNA Methylation Analyses Reveal Potential Sex Determination/Differentiation Mechanisms of the Deep-Sea Mussel Gigantidas platifrons
文献类型：期刊论文
| 作者 | Zhong, Zhaoshan1,2,3 ; Wang, Minxiao1,2,3; Chen, Hao1,2,3; Wang, Hao1,2,3; Zhang, Huan1,2,3; Zhou, Li1,2,3; Sun, Yan1,2,3; Cao, Lei1,2,3; Lian, Chao1,2,3; Li, Mengna1,2,3,4
|
| 刊名 | FRONTIERS IN MARINE SCIENCE
 |
| 出版日期 | 2022-04-28 |
| 卷号 | 9页码:15 |
| 关键词 | deep-sea mussel Gigantidas platifrons gonad transcriptome methylation sex determination and differentiation |
| DOI | 10.3389/fmars.2022.856291 |
| 通讯作者 | Li, Chaolun(lcl@qdio.ac.cn) |
| 英文摘要 | Gigantidas platifrons is one of the most dominant deep-sea mussels in cold seeps ecosystems in the South China sea. Studies have shown that deep-sea mussels are a gonochoristic species, however, little is known about the molecular mechanisms of sex determination in G. platifrons. In this study, RNA-seq and WGBS methylation analysis were performed on adult G. platifrons gonads to identify potential sex-related genes and generate a comprehensive analysis of sex determination in deep-sea mussels. A total of 5923 genes were identified as differentially expressed between the ovaries and testes, of which 2711 were female-biased and 3212 were male-biased. Among them, 161 genes may participate in the sex determination, and we found that DMRT2 may play an important role in male sex determination, and FOXL2, Wnt7, and beta-catenin may have impact on female sex determination. Moreover, common expression patterns were found in majority of the sex-related genes such as FOXL2, beta-catenin, and genes in SOX family, suggesting the sex determination mechanisms of mussels in different habitats were conserved. The 5mC levels of transcription start sites (TSS2K) were significantly higher in sex-related genes than other DEGs in both gonads. Positive correlation was observed between sex-related genes expression and methylation in male, however, the effects of the DNA methylation on gene expression were complex in female. In short, we argue that sex determination mechanisms of deep-sea mussel G. platifrons is mainly controlled by genetic, and the methylation may have a regulation role on male sex determination or differentiation. |
| WOS研究方向 | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| 出版者 | FRONTIERS MEDIA SA |
| WOS记录号 | WOS:000794553600001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/179224]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Li, Chaolun |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Ctr Deep Sea Res, Qingdao, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhong, Zhaoshan,Wang, Minxiao,Chen, Hao,et al. Gonad Transcriptome and Whole-Genome DNA Methylation Analyses Reveal Potential Sex Determination/Differentiation Mechanisms of the Deep-Sea Mussel Gigantidas platifrons[J]. FRONTIERS IN MARINE SCIENCE,2022,9:15. |
| APA | Zhong, Zhaoshan.,Wang, Minxiao.,Chen, Hao.,Wang, Hao.,Zhang, Huan.,...&Li, Chaolun.(2022).Gonad Transcriptome and Whole-Genome DNA Methylation Analyses Reveal Potential Sex Determination/Differentiation Mechanisms of the Deep-Sea Mussel Gigantidas platifrons.FRONTIERS IN MARINE SCIENCE,9,15. |
| MLA | Zhong, Zhaoshan,et al."Gonad Transcriptome and Whole-Genome DNA Methylation Analyses Reveal Potential Sex Determination/Differentiation Mechanisms of the Deep-Sea Mussel Gigantidas platifrons".FRONTIERS IN MARINE SCIENCE 9(2022):15. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。