Co-occurrence of Alexandrium minutum (Dinophyceae) ribotypes from the Chinese and Malaysian coastal waters and their toxin production
文献类型：期刊论文
| 作者 | Liu, Minlu1; Krock, Bernd2; Yu, Rencheng6; Leaw, Chui Pin5; Lim, Po Teen5; Ding, Guangmao4; Wang, Na1; Zheng, Jing1; Gu, Haifeng1,3,7 |
| 刊名 | HARMFUL ALGAE
 |
| 出版日期 | 2022-06-01 |
| 卷号 | 115页码:14 |
| ISSN号 | 1568-9883 |
| 关键词 | Cryptic species Dinoflagellates Harmful algal bloom (HAB) Metabarcoding Paralytic shellfish toxins (PSTs) |
| DOI | 10.1016/j.hal.2022.102238 |
| 通讯作者 | Gu, Haifeng(guhaifeng@tio.org.cn) |
| 英文摘要 | The bloom-forming dinophyte Alexandrium minutum comprises biogeographic inferred, global and Pacific clades with both toxic and nontoxic strains reported. A. minutum has a wide distribution in the Western Pacific, but to date only a few strains have available DNA sequences. To fully understand its genetic diversity, sampling was undertaken from the Yellow Sea, the East and South China Sea, and five strains of A. minutum and two strains of its sister species, A. tamutum, were established. Their morphology was examined by light and scanning electron microscopy. In addition, sequences were obtained from both large subunit (LSU) ribosomal DNA and/or internal transcribed spacer (ITS) region. Strains of A. minutum are morphologically indistinguishable, characterized by a smaller cell size and a narrow sixth precingular plate. In contrast, A. tamutum has a wider sixth precingular plate. High nucleotide divergences of LSU (D1-D3) rDNA and ITS were revealed amongst strains of A. minutum (10% and 25%, respectively), and A. tamutum (3% and 13%, respectively). Molecular phylogenies based on LSU rDNA and ITS revealed three ribotypes (B-D) of A. minutum, and two ribotypes of A. tamutum in the Western Pacific. Seasonal sampling in the East China Sea to detect A. minutum using the DNA metabarcoding targeting ITS1 region was also performed. Our results showed that the ribotypes B and C of A. minutum co-occurred in the water. Paralytic shellfish toxin (PSTs) of all seven strains was analysed using liquid chromatography with tandem mass spectrometry (LC-MS/MS). PSTs were detected only in A. minutum ribotypes B and C with predominance of gonyautoxins 1/4. Our results suggest high diversity and risk potential of this toxic species in this region. |
| 资助项目 | National Natural Science Foundation of China[42030404] ; National Key Research and Development Program of China[2019YFE0124700] ; Fund Project for Marine and Fishery Protection and Development in Fujian Province[FZJZ-2021-1] ; Ministry of Higher Education of Malaysia HICOE ; [LRGS/1/2020/UMT/01/1/3] |
| WOS研究方向 | Marine & Freshwater Biology |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000798742200001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/179383]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Gu, Haifeng |
| 作者单位 | 1.Minist Nat Resources, Inst Oceanog 3, Xiamen 361005, Peoples R China 2.Helmholtz Zent Polar & Meeresforschung, Alfred Wegener Inst, Handelshafen 12, D-27570 Bremerhaven, Germany 3.Minist Nat Resources, Key Lab Marine Ecol Conservat & Restorat, Xiamen 361005, Peoples R China 4.Fishery Resources Monitoring Ctr Fujian Prov, Fuzhou 350003, Peoples R China 5.Univ Malaya, Inst Ocean & Earth Sci, Bachok Marine Res Stn, Bachok 16310, Kelantan, Malaysia 6.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 7.Fujian Prov Key Lab Marine Ecol Conservat & Restor, Xiamen 361005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Minlu,Krock, Bernd,Yu, Rencheng,et al. Co-occurrence of Alexandrium minutum (Dinophyceae) ribotypes from the Chinese and Malaysian coastal waters and their toxin production[J]. HARMFUL ALGAE,2022,115:14. |
| APA | Liu, Minlu.,Krock, Bernd.,Yu, Rencheng.,Leaw, Chui Pin.,Lim, Po Teen.,...&Gu, Haifeng.(2022).Co-occurrence of Alexandrium minutum (Dinophyceae) ribotypes from the Chinese and Malaysian coastal waters and their toxin production.HARMFUL ALGAE,115,14. |
| MLA | Liu, Minlu,et al."Co-occurrence of Alexandrium minutum (Dinophyceae) ribotypes from the Chinese and Malaysian coastal waters and their toxin production".HARMFUL ALGAE 115(2022):14. |
入库方式： OAI收割
来源：海洋研究所
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