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Facile fabrication of highly sensitive and non-label aptasensors based on antifouling amyloid-like protein aggregates

文献类型：期刊论文

作者Ye, Xiangyi2,3; Zhang, Dun1,2,3; Zeng, Yan2,3; Wang, Yingwen1,2,3; Qi, Peng1,2,3
刊名ANALYTICAL METHODS
出版日期2022-05-11
页码7
ISSN号1759-9660
DOI10.1039/d2ay00416j
通讯作者Zhang, Dun(zhangdun@qdio.ac.cn) ; Qi, Peng(qipeng@qdio.ac.cn)
英文摘要In this paper, we present a robust and versatile method for developing non-label aptasensors with high sensitivity. Amyloid-like protein aggregates were facilely synthesized with the commonly used passivating agent bovine serum albumin (BSA) in developing biosensors, and the produced amyloid-like phase-transited BSA (PTB) exhibited excellent antifouling performances and robust interfacial adhesion with the electrode surface. In order to improve the detection sensitivity of electrochemical measurements, reduced graphene oxide was electrochemically deposited onto the electrode surface. Moreover, gold nanoparticles were introduced to enhance the conductivity of the PTB film and facilitate subsequent aptamer modification. Two common biological species, adenosine triphosphate (ATP) and cytochrome c (cyt c), were chosen as detection targets, and their corresponding aptasensors were successfully constructed and systematically evaluated. The proposed aptasensors based on the PTB-Au antifouling composite exhibited high sensitivity and specificity towards ATP and cyt c detection, and the detection limits were calculated to be 0.26 nM and 0.64 nM for ATP and cyt c, respectively. Hence, this work provides a simple approach to develop highly sensitive aptasensors without any labeling process, and thus promises its great application in biological analysis.
资助项目National Natural Science Foundation of China[41876101] ; National Natural Science Foundation of China[41906037] ; Nantong Scientific Plan Foundation[JC2021054] ; Nantong Scientific Plan Foundation[JC2020090]
WOS研究方向Chemistry ; Food Science & Technology ; Spectroscopy
语种英语
WOS记录号WOS:000804237200001
出版者ROYAL SOC CHEMISTRY
源URL[http://ir.qdio.ac.cn/handle/337002/179410]  
专题海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者Zhang, Dun; Qi, Peng
作者单位1.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China
3.Pilot Natl Lab Marine Sci & Technol Qingdao, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China
推荐引用方式
GB/T 7714		 Ye, Xiangyi,Zhang, Dun,Zeng, Yan,et al. Facile fabrication of highly sensitive and non-label aptasensors based on antifouling amyloid-like protein aggregates[J]. ANALYTICAL METHODS,2022:7.
APA Ye, Xiangyi,Zhang, Dun,Zeng, Yan,Wang, Yingwen,&Qi, Peng.(2022).Facile fabrication of highly sensitive and non-label aptasensors based on antifouling amyloid-like protein aggregates.ANALYTICAL METHODS,7.
MLA Ye, Xiangyi,et al."Facile fabrication of highly sensitive and non-label aptasensors based on antifouling amyloid-like protein aggregates".ANALYTICAL METHODS (2022):7.

入库方式： OAI收割

来源：海洋研究所

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