Aptamer-based Cas14a1 biosensor for amplification-free live pathogenic detection
文献类型：期刊论文
| 作者 | Wei, Yangdao3; Tao, Zhenzhen3; Wan, Lu3; Zong, Chengli3; Wu, Jiajia1 ; Tan, Xiao2; Wang, Buhua3; Guo, Zixuan3; Zhang, Ling3; Yuan, Haoyu3
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| 刊名 | BIOSENSORS & BIOELECTRONICS
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| 出版日期 | 2022-09-01 |
| 卷号 | 211页码:6 |
| ISSN号 | 0956-5663 |
| 关键词 | CRISPR-Cas Bacteria diagnostic Staphylococcus aureus |
| DOI | 10.1016/j.bios.2022.114282 |
| 通讯作者 | Yang, Zhiqing(yangzhiqing@hainanu.edu.cn) ; Wan, Yi(993602@hainanu.edu.cn) |
| 英文摘要 | CRISPR-Cas systems have been employed to detect a large variety of pathogenic microorganisms by simply changing the guide RNA sequence. However, these platforms usually rely on nucleic acid extraction and amplification to achieve good sensitivity. Herein, we developed a new platform for the highly specific and sensitive detection of live staphylococcus aureus (S. aureus) based on an Aptamer-based Cas14a1 Biosensor (ACasB), without the need for nucleic acid extraction or amplification. First, the S. aureus specific aptamer was hybrid with a blocker DNA. After the live S. aureus was added, the blocker can be released upon bacteria-aptamer binding. Finally, the released blocker can activate Cas14a1 protein by binding with the sgRNA to generate a change of fluorescent intensity. The ACasB indicates high specificity and sensitivity: it can directly distinguish 400 CFU/ml live S. aureus cells. Comparable to qPCR, the Cas14a1-aptamer biosensor can detect S. aureus with 100% accuracy in complex samples. Therefore, this ACasB for the on-site detection of live S. aureus can broaden its applications in food safety and environmental monitoring. |
| 资助项目 | Hainan Provincial Natural Science Foundation of China, China[2019RC092] ; Hainan Provincial Natural Science Foundation of China, China[420RC518] ; Hainan Provincial Natural Science Foundation of China, China[821CXTD1011] ; National Natural Science Foundation of China, China[41866002] ; National Natural Science Foundation of China, China[4216060011] ; National Natural Science Foundation of China, China[82073697] ; Open Fund of Shandong Key Laboratory of Corrosion Science, China[KLCS201910] ; Research Foundation of Hainan University, China[KYQD (ZR) 1711] ; Research Foundation of Hainan University, China[KYQD (ZR) 1997] ; Research Foundation of Hainan University, China[KYQD (ZR) 20087] ; Open fund of Wenzhou Key Laboratory of Sanitary Microbiology, China[ZD202003KF04] ; Open fund of Fujian Provincial Key Laboratory of Photonics Technology, China[JYG 2002] |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| WOS记录号 | WOS:000806344000003 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/179437]  |
| 专题 | 海洋研究所_海洋腐蚀与防护研究发展中心 |
| 通讯作者 | Yang, Zhiqing; Wan, Yi |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 2.Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China 3.Hainan Univ, Sch Pharmaceut Sci, State Key Lab Marine Resource Utilizat South Chin, Haikou 570228, Hainan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Yangdao,Tao, Zhenzhen,Wan, Lu,et al. Aptamer-based Cas14a1 biosensor for amplification-free live pathogenic detection[J]. BIOSENSORS & BIOELECTRONICS,2022,211:6. |
| APA | Wei, Yangdao.,Tao, Zhenzhen.,Wan, Lu.,Zong, Chengli.,Wu, Jiajia.,...&Wan, Yi.(2022).Aptamer-based Cas14a1 biosensor for amplification-free live pathogenic detection.BIOSENSORS & BIOELECTRONICS,211,6. |
| MLA | Wei, Yangdao,et al."Aptamer-based Cas14a1 biosensor for amplification-free live pathogenic detection".BIOSENSORS & BIOELECTRONICS 211(2022):6. |
入库方式： OAI收割
来源：海洋研究所
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