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Fluorescence imaging to probe mercury induced oxidative stress in living systems

文献类型：期刊论文

作者Zhang, Xia2,6; Zhang, Li2,6; Cheng, Xinyan5; Liu, Shudi5; Fang, Shujing5; Zhang, Liangwei4,6; Wang, Xiaoyan1; Chen, Lingxin3,4,6
刊名SENSORS AND ACTUATORS B-CHEMICAL
出版日期2022-09-01
卷号366页码:8
关键词Fluorescent imaging Mercury stress Oxidative stress Hydrogen peroxide Hypoxia
DOI10.1016/j.snb.2022.131982
通讯作者Zhang, Liangwei(liangweizhang@yic.ac.cn) ; Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要Mercury is known a toxic heavy metal pollutant, and mercuric derivatives severely threaten ecological environment and human health. Their toxicological effects are usually associated with oxidative stress and redox imbalance. However, rare visualized evidences to reveal mercury induced oxidative stress in living systems. According to its characteristics, we suspected mercury resulted in two consequences, one is reactive oxygen species (ROS) outburst, the other is oxygen consumption caused hypoxia stress. Hydrogen peroxide (H2O2) is an important oxygen metabolite, and superfluous H2O2 will attack intracellular antioxidants and trigger oxidative stress, then cause dysfunctions and many diseases. Although the research of H2O2 fluorescence probe has been greatly advanced, the exploration of H2O2 bioeffects under environmental stress was scarce. There is still necessary to develop powerful tools to explore redox homeostasis and H2O2 fluctuations under mercury stress. Herein, we have designed fluorescent probe MSO-Bindol for monitoring H2O2 fluctuations through destruction of carbon-carbon double bond leading to ratiometric fluorescent changes. MSO-Bindol is successfully used to observe H2O2 variations in cells and zebrafish exposed to various stimulations or environmental stress especially mercury stress. Moreover, imaging results provide visualization proofs for Hg2+-mediated H2O2 variations and reveal the relationship between Hg2+-induced oxidative stress and up-regulated H2O2 in biological systems.
WOS关键词HYDROGEN-PEROXIDE ; REACTIVE OXYGEN ; H2O2 ; MITOCHONDRIA ; SIGNALS ; CELLS
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种英语
WOS记录号WOS:000800383700004
资助机构National Natural Science Foundation of China ; Program of Youth Innovation Promotion Association, CAS ; Taishan Scholar Project Special Funding
源URL[http://ir.yic.ac.cn/handle/133337/31119]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
烟台海岸带研究所_山东省海岸带环境工程技术研究中心
通讯作者Zhang, Liangwei; Chen, Lingxin
作者单位1.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
5.Yantai Univ, Coll Chem & Chem Engn, Yantai 264005, Peoples R China
6.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Xia,Zhang, Li,Cheng, Xinyan,et al. Fluorescence imaging to probe mercury induced oxidative stress in living systems[J]. SENSORS AND ACTUATORS B-CHEMICAL,2022,366:8.
APA Zhang, Xia.,Zhang, Li.,Cheng, Xinyan.,Liu, Shudi.,Fang, Shujing.,...&Chen, Lingxin.(2022).Fluorescence imaging to probe mercury induced oxidative stress in living systems.SENSORS AND ACTUATORS B-CHEMICAL,366,8.
MLA Zhang, Xia,et al."Fluorescence imaging to probe mercury induced oxidative stress in living systems".SENSORS AND ACTUATORS B-CHEMICAL 366(2022):8.

入库方式： OAI收割

来源：烟台海岸带研究所

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