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Potentiometric aptasensing of Escherichia coli based on electrogenerated chemiluminescence as a highly sensitive readout

文献类型：期刊论文

作者Gao, Xueqing1,4; Jiang, Tianjia1; Qin, Wei1,2,3
刊名BIOSENSORS & BIOELECTRONICS
出版日期2022-03-15
卷号200页码:7
关键词Potentiometry Aptasensor Electrogenerated chemiluminescence Escherichia coli
ISSN号0956-5663
DOI10.1016/j.bios.2021.113923
通讯作者Jiang, Tianjia(tjjiang@yic.ac.cn) ; Qin, Wei(wqin@yic.ac.cn)
英文摘要We introduce here a versatile approach to read out potentiometric aptasensors by electrogenerated chemiluminescence (ECL), which can amplify the small potential changes induced by the bacterial concentrations via ECL signals. In the present system, the electrode modified with single-walled carbon nanotubes (SWCNTs) and aptamer molecules acts as the reference electrode and is placed in the sample solution for sensing the bacterial concentration changes, while the Ru(bpy)(3)(2+) modified gold electrode serves as the working electrode for generating ECL signals and is placed in the detection solution containing tripropylamine (TPA) spatially separated from the sample solution by a salt bridge. Ru(bpy)(3)(2+) is immobilized on the gold electrode's surface for enhancement of luminous efficiency and reduction of reagent consumption. A moving-part-free fluid flowing system is introduced to promote the mass transport of TPA from the detection solution to the surface of the ECL generating electrode. When a constant potential is imposed between the working and reference electrodes, the potential changes at the SWCNTs-aptamer modified electrode induced by the bacterial concentrations can modulate the potentials at the Ru(bpy)(3)(2+) modified electrode, thus generating the ECL signals. The developed sensing strategy shows a highly sensitive response to E. coli O157: H7 in the linear range of 5-1000 CFU mL(-1) with a low detection limit of 2 CFU mL(-1). We believe that the proposed approach is promising to develop aptasensors for sensitive detection of bacterial cells.
WOS关键词WALLED CARBON NANOTUBES ; IMMEDIATE DETECTION ; BACTERIA ; ELECTROCHEMILUMINESCENCE ; RELEASE ; SYSTEM
WOS研究方向Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种英语
WOS记录号WOS:000772286100009
资助机构National Natural Science Foundation of China ; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences ; Taishan Scholar Program of Shandong Province
源URL[http://ir.yic.ac.cn/handle/133337/31357]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
烟台海岸带研究所_近岸生态与环境实验室
通讯作者Jiang, Tianjia; Qin, Wei
作者单位1.Chinese Acad Sci, Shandong Key Lab Coastal Environm Proc, CAS Key Lab Coastal Environm Proc & Ecol Remediat, YICCAS,Yantai Inst Coastal Zone Res YIC, Yantai 264003, Shandong, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Gao, Xueqing,Jiang, Tianjia,Qin, Wei. Potentiometric aptasensing of Escherichia coli based on electrogenerated chemiluminescence as a highly sensitive readout[J]. BIOSENSORS & BIOELECTRONICS,2022,200:7.
APA Gao, Xueqing,Jiang, Tianjia,&Qin, Wei.(2022).Potentiometric aptasensing of Escherichia coli based on electrogenerated chemiluminescence as a highly sensitive readout.BIOSENSORS & BIOELECTRONICS,200,7.
MLA Gao, Xueqing,et al."Potentiometric aptasensing of Escherichia coli based on electrogenerated chemiluminescence as a highly sensitive readout".BIOSENSORS & BIOELECTRONICS 200(2022):7.

入库方式： OAI收割

来源：烟台海岸带研究所

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