Simultaneous quantitative analysis of seven steroid hormones in human saliva: A novel method based on O-ethylhydroxylamine hydrochloride as derivatization reagent
文献类型：期刊论文
| 作者 | Xu, Bin1; Jia, Pengfei1; Cai, Jun1; Gu, Lidong1; Yan, Huaxiao2; Zhao, Hui2; Qin, Song2,3
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| 刊名 | RAPID COMMUNICATIONS IN MASS SPECTROMETRY
 |
| 出版日期 | 2022-04-30 |
| 卷号 | 36期号:8页码:10 |
| ISSN号 | 0951-4198 |
| DOI | 10.1002/rcm.9242 |
| 通讯作者 | Cai, Jun(caijun068@163.com) |
| 英文摘要 | Rationale Saliva has been widely accepted as a more convenient alternative to serum or plasma in the field of clinical diagnosis. However, the detection of trace components in saliva has been a bottleneck problem. The aim of this work was to develop a highly sensitive and reliable method for simultaneously determining the trace steroid hormones including some with poor ionization efficiency in human saliva by liquid chromatography/tandem mass spectrometry (LC/MS). Methods Saliva was deproteinated by acetonitrile containing mixed isotope internal standards and extracted with methyl tert-butyl ether. The extraction solution was dried under a stream of nitrogen and the residue was derivatized using 50 mM O-ethylhydroxylamine hydrochloride in 80% methanol/water solution (v/v). The processed sample was determined by LC/MS in multiple reaction monitoring (MRM) mode. Results The method was successfully established for the simultaneous quantification of seven steroid hormones in human saliva and showed excellent specificity and sensitivity. The limits of quantification (LOQs) of all steroid hormones were below 5 pg/mL, in particular, the LOQ of progesterone was as low as 0.15 pg/mL. The linear correlation coefficients (r) were greater than 0.9990 in the range of 2-200 pg/mL for T, DHEA, A4, P4, P5, and 17OHP4 and in the range of 5-500 pg/mL for 17OHP5. The intra-day and inter-day variability ranged from 1.86% to 7.83% and 1.95% to 10.4%, respectively. The recovery of the method ranged from 86.9% to 111.1% for all steroid hormones using three spiked concentrations. Conclusions A novel LC/MS/MS method was developed for the simultaneous quantification of seven kinds of trace steroid hormones in human saliva. The results of the methodological study showed that the method exhibited excellent sensitivity and reliability for the evaluation of free steroid hormones in the human body. It is believed that this method could provide useful information of steroid hormone metabolism for auxiliary diagnosis of some endocrine disorders. |
| WOS关键词 | MASS-SPECTROMETRY ; LC-MS/MS ; CORTISOL ; DEHYDROEPIANDROSTERONE ; SERUM ; TOOL |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000774173300007 |
| 资助机构 | National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province ; Foundation of SINOPEC ; Major Innovation Projects of Key R&D Program of Shandong Province ; Qingdao National Laboratory for Marine Science and Technology ; SDUST Research Fund |
| 源URL | [http://ir.yic.ac.cn/handle/133337/31354]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 通讯作者 | Cai, Jun |
| 作者单位 | 1.Excellent Technol Co Ltd, Suzhou 215000, Peoples R China 2.Shandong Univ Sci & Technol, Coll Chem & Biol Engn, Shandong Innovat Ctr Nongrain Ethanol Biorefinery, Dept Bioengn, Qingdao, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Bin,Jia, Pengfei,Cai, Jun,et al. Simultaneous quantitative analysis of seven steroid hormones in human saliva: A novel method based on O-ethylhydroxylamine hydrochloride as derivatization reagent[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2022,36(8):10. |
| APA | Xu, Bin.,Jia, Pengfei.,Cai, Jun.,Gu, Lidong.,Yan, Huaxiao.,...&Qin, Song.(2022).Simultaneous quantitative analysis of seven steroid hormones in human saliva: A novel method based on O-ethylhydroxylamine hydrochloride as derivatization reagent.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,36(8),10. |
| MLA | Xu, Bin,et al."Simultaneous quantitative analysis of seven steroid hormones in human saliva: A novel method based on O-ethylhydroxylamine hydrochloride as derivatization reagent".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 36.8(2022):10. |
入库方式： OAI收割
来源：烟台海岸带研究所
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