Functional Metabolomics Reveals that Astragalus Polysaccharides Improve Lipids Metabolism through Microbial Metabolite 2-Hydroxybutyric Acid in Obese Mice
文献类型：期刊论文
| 作者 | Li, Bingbing5; Hong, Ying5; Gu, Yu5; Ye, Shengjie5; Hu, Kaili5; Yao, Jian4; Ding, Kan4 ; Zhao, Aihua2,3; Jia, Wei1,2,3; Li, Houkai5
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| 刊名 | ENGINEERING
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| 出版日期 | 2022-02-01 |
| 卷号 | 9页码:111-122 |
| ISSN号 | 2095-8099 |
| 关键词 | Astragalus polysaccharides (APS) Functional metabolomics 2-Hydroxybutyric acid Obesity |
| DOI | 10.1016/j.eng.2020.05.023 |
| 通讯作者 | Li, Houkai(houkai1976@126.com) |
| 英文摘要 | Polysaccharides are widely present in herbs with multiple activities, especially immunity regulation and metabolic benefits for metabolic disorders. However, the underlying mechanisms are not well understood. Functional metabolomics is increasingly used to investigate systemic effects on the host by identifying metabolites with particular functions. This study explores the mechanisms underlying the metabolic benefits of Astragalus polysaccharides (APS) by adopting a functional metabolomics strategy. The effects of APS were determined in eight-week high-fat diet (HFD)-fed obese mice. Then, gas chromatography-time-of-flight mass spectrometry (GC-TOFMS)-based untargeted metabolomics was performed for an analysis of serum and liver tissues, and liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based targeted metabolomics was performed. The potential functions of the metabolites were tested with in vitro and in vivo models of metabolic disorders. Our results first confirmed the metabolic benefits of APS in obese mice. Then, metabolomics analysis revealed that APS supplementation reversed the HFD-induced metabolic changes, and identified 2-hydroxybutyric acid (2-HB) as a potential functional metabolite for APS activity that was significantly decreased by a HFD and reversed by APS. Further study indicated that 2-HB inhibited oleic acid (OA)-induced triglyceride (TG) accumulation. It was also found to stimulate the expression of proteins in lipid degradation in hepatocytes and TG lipolysis in 3T3-L1 cells. Moreover, it was found to reduce serum TG and regulate the proteins involved in lipid degradation in high-fat and high-sucrose (HFHS)-fed mice. In conclusion, our study demonstrates that the metabolic benefits of APS are at least partially due to 2-HB generation, which modulated lipid metabolism both in vitro and in vivo. Our results also highlight that functional metabolomics is practical for investigating the mechanism underlying the systemic benefits of plant polysaccharides.(c) 2021 THE AUTHORS. Published by Elsevier LTD on behalf of Chinese Academy of Engineering and Higher Education Press Limited Company. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| WOS关键词 | GENOME SEQUENCE ; FATTY-ACIDS ; INSULIN-RESISTANCE ; HEPATIC STEATOSIS ; INFLAMMATION ; DIET ; DEFICIENCY ; INHIBITION ; INDUCTION ; LIPOLYSIS |
| 资助项目 | National Natural Science Foundation of China[81673662] ; National Natural Science Foundation of China[81873059] ; Program for Professor of Special Appointment (Eastern Scholar) & Shuguang Scholar at Shanghai Institutions of Higher Learning from Shanghai Municipal Education[16SG36] |
| WOS研究方向 | Engineering |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000791266000018 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/301015]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Li, Houkai |
| 作者单位 | 1.Univ Hawaii, Canc Ctr, Honolulu, HI 96813 USA 2.Shanghai Jiao Tong Univ Affiliated Peoples Hosp 6, Ctr Translat Med, Shanghai 200233, Peoples R China 3.Shanghai Jiao Tong Univ Affiliated Peoples Hosp 6, Shanghai Key Lab Diabet Mellitus, Shanghai 200233, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 5.Shanghai Univ Tradit Chinese Med, Inst Interdisciplinary Integrat Med Res, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Bingbing,Hong, Ying,Gu, Yu,et al. Functional Metabolomics Reveals that Astragalus Polysaccharides Improve Lipids Metabolism through Microbial Metabolite 2-Hydroxybutyric Acid in Obese Mice[J]. ENGINEERING,2022,9:111-122. |
| APA | Li, Bingbing.,Hong, Ying.,Gu, Yu.,Ye, Shengjie.,Hu, Kaili.,...&Li, Houkai.(2022).Functional Metabolomics Reveals that Astragalus Polysaccharides Improve Lipids Metabolism through Microbial Metabolite 2-Hydroxybutyric Acid in Obese Mice.ENGINEERING,9,111-122. |
| MLA | Li, Bingbing,et al."Functional Metabolomics Reveals that Astragalus Polysaccharides Improve Lipids Metabolism through Microbial Metabolite 2-Hydroxybutyric Acid in Obese Mice".ENGINEERING 9(2022):111-122. |
入库方式： OAI收割
来源：上海药物研究所
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