Salvianolate ameliorates renal tubular injury through the Keap1/Nrf2/ARE pathway in mouse kidney ischemia-reperfusion injury
文献类型：期刊论文
| 作者 | Sun, Dan5,6; Cui, Shichao5; Ma, Haijian4; Zhu, Pengfei3; Li, Ni5; Zhang, Xinwen5; Zhang, Lina2; Xuan, Lijiang5 ; Li, Jingya1,5,6
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| 刊名 | JOURNAL OF ETHNOPHARMACOLOGY
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| 出版日期 | 2022-07-15 |
| 卷号 | 293页码:10 |
| 关键词 | Acute kidney injury Salvianolate Apoptosis Oxidative stress Keap1-Nrf2-ARE |
| ISSN号 | 0378-8741 |
| DOI | 10.1016/j.jep.2022.115331 |
| 通讯作者 | Li, Jingya(jyli@simm.ac.cn) |
| 英文摘要 | Ethnopharmacological relevance: Acute kidney injury (AKI) is a common clinical disease characterized by rapid loss of renal function. Salvianolate is a prescribed Chinese medicine derived from traditional Chinese medicine Salvia miltiorrhiza bunge that possesses many pharmacological effects, the active components extracted from Salvia miltiorrhiza bunge have been proved to protect ischemia-reperfusion (I/R)-AKI. Aim of the study: This study aims to validate the therapeutic effect of SAL on I/R-AKI, and explore its potential pharmacological mechanism. Materials and methods: Mice were pretreated with/without salvianolate (10, 30, and 90 mg/kg) before renal ischemia-reperfusion operation. Serum creatinine, BUN, and H&E staining were performed to evaluate renal function. Immunofluorescence analysis was conducted to measure renal tubular injury including inflammatory factors and peroxide level. Apoptosis of the kidney tissues was determined by TUNEL assay. Keap1-Nrf2-ARE and apoptosis signaling pathways were measured by Western blot, RT-PCR, and YO-PRO-1 staining in kidneys or NRK52E cells. Results: Pretreatment with SAL effectively alleviated renal function and ameliorated epithelial tubular injury, oxidative stress, and inflammatory response. Furthermore, the mechanistic study demonstrated that the SAL exerts anti-apoptotic effects through activation of the Keap1-Nrf2-ARE signaling pathway in renal tubular cells. Conclusion: These findings indicate the therapeutic benefit of salvianolate in the protection of renal injury from ischemia-reperfusion, and strengthen the evidence for the AKI treatment strategy by the anti-oxidative stress response, suggesting that SAL may be a potential agent for the treatment of AKI. |
| WOS关键词 | TRANSCRIPTION FACTOR NRF2 ; KEAP1-NRF2 SYSTEM ; ACID ; ACTIVATION ; FIBROSIS ; STRESS ; MODEL ; AKI |
| 资助项目 | Shanghai Commission of Science and Technology[21S11907600] ; Shanghai Commission of Science and Technology[TM202101H00] |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000797939100003 |
| 出版者 | ELSEVIER IRELAND LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/301092]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Li, Jingya |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, 189 Guo Shou Jing Rd, Shanghai 201203, Peoples R China 2.Shanghai Green Valley Pharmaceut Co Ltd, Shanghai, Peoples R China 3.Nanjing Univ Chinese Med, Clin Med Sch 1, Nanjing, Peoples R China 4.Fudan Univ, Sch Pharm, Dept Pharmacol, Shanghai, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China 6.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Dan,Cui, Shichao,Ma, Haijian,et al. Salvianolate ameliorates renal tubular injury through the Keap1/Nrf2/ARE pathway in mouse kidney ischemia-reperfusion injury[J]. JOURNAL OF ETHNOPHARMACOLOGY,2022,293:10. |
| APA | Sun, Dan.,Cui, Shichao.,Ma, Haijian.,Zhu, Pengfei.,Li, Ni.,...&Li, Jingya.(2022).Salvianolate ameliorates renal tubular injury through the Keap1/Nrf2/ARE pathway in mouse kidney ischemia-reperfusion injury.JOURNAL OF ETHNOPHARMACOLOGY,293,10. |
| MLA | Sun, Dan,et al."Salvianolate ameliorates renal tubular injury through the Keap1/Nrf2/ARE pathway in mouse kidney ischemia-reperfusion injury".JOURNAL OF ETHNOPHARMACOLOGY 293(2022):10. |
入库方式： OAI收割
来源：上海药物研究所
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