Protective effect of mesenchymal stem cell-derived exosomal treatment of hippocampal neurons against oxygen-glucose deprivation/reperfusion-induced injury
文献类型：期刊论文
| 作者 | Guo, Xiao-fang4; Gu, Shuang-shuang4; Wang, Jun3; Sun, Hao4; Zhang, Yu-juan2; Yu, Peng-fei1; Zhang, Jin-song4; Jiang, Lei4 |
| 刊名 | WORLD JOURNAL OF EMERGENCY MEDICINE
 |
| 出版日期 | 2022 |
| 卷号 | 13期号:1页码:46-53 |
| ISSN号 | 1920-8642 |
| 关键词 | Mesenchymal stem cells Exosomes Oxygen-glucose deprivation reperfusion Reactive oxygen species Mitochondria |
| DOI | 10.5847/wjem.j.1920-8642.2022.015 |
| 通讯作者 | Zhang, Jin-song(zhangjso@njmu.edu.cn) ; Jiang, Lei(racheljl@126.com) |
| 英文摘要 | BACKGROUND: Individuals who survive a cardiac arrest often sustain cognitive impairments due to ischemia-reperfusion injury. Mesenchymal stem cell (MSC) transplantation is used to reduce tissue damage, but exosomes are more stable and highly conserved than MSCs. This study was conducted to investigate the therapeutic eff ects of MSC-derived exosomes (MSC-Exo) on cerebral ischemia-reperfusion injury in an in vitro model of oxygen-glucose deprivation/reperfusion (OGD/R), and to explore the underlying mechanisms. METHODS: Primary hippocampal neurons obtained from 18-day Sprague-Dawley rat embryos were subjected to OGD/R treatment, with or without MSC-Exo treatment. Exosomal integration, cell viability, mitochondrial membrane potential, and generation of reactive oxygen species (ROS) were examined. Terminal deoxynucleotidyl transferase-mediated 2???-deoxyuridine 5???-triphosphate nick -end labeling (TUNEL) staining was performed to detect neuronal apoptosis. Moreover, mitochondrial function-associated gene expression, Nrf2 translocation, and expression of downstream antioxidant proteins were determined. RESU LTS: MSC-Exo attenuated OGD/R-induced neuronal apoptosis and decreased ROS generation (P<0.05). The exosomes reduced OGD/R-induced Nrf2 translocation into the nucleus (2.14??0.65 vs. 5.48??1.09, P <0.01) and increased the intracellular expression of antioxidative proteins, including superoxide dismutase and glutathione peroxidase (17.18??0.97 vs. 14.40??0.62, and 20.65??2.23 vs. 16.44??2.05, respectively; P <0.05 for both). OGD/R significantly impaired the mitochondrial membrane potential and modulated the expression of mitochondrial function-associated genes, such as PINK, DJ1, LRRK2, Mfn-1, Mfn-2, and OPA1. The abovementioned changes were partially reversed by exosomal treatment of the hippocampal neurons. CONCLUSIONS: MSC-Exo treatment can alleviate OGD/R-induced oxidative stress and dysregulation of mitochondrial function-associated genes in hippocampal neurons. Therefore, MSC-Exo might be a potential therapeutic strategy to prevent OGD/R-induced neuronal injury. |
| WOS关键词 | EXTRACELLULAR VESICLES ; MITOCHONDRIA ; NRF2 |
| 资助项目 | National Natural Science Foundation of China[81701872] |
| WOS研究方向 | Emergency Medicine |
| 语种 | 英语 |
| 出版者 | ZHEJIANG UNIV PRESS |
| WOS记录号 | WOS:000804740100008 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/301231]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zhang, Jin-song; Jiang, Lei |
| 作者单位 | 1.Shanghai Inst Mat Med, Ctr Drug Safety Evaluat & Res, Shanghai 201203, Peoples R China 2.Nanjing Med Univ, Sch Publ Hlth, Dept Environm Genom, Key Lab Modern Toxicol,Minist Educ, Nanjing 211166, Peoples R China 3.Nanjing Med Univ, Sch Publ Hlth, Dept Toxicol, Key Lab Modern Toxicol,Minist Educ, Nanjing 211166, Peoples R China 4.Nanjing Med Univ, Dept Emergency, Affiliated Hosp 1, Nanjing 210029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Xiao-fang,Gu, Shuang-shuang,Wang, Jun,et al. Protective effect of mesenchymal stem cell-derived exosomal treatment of hippocampal neurons against oxygen-glucose deprivation/reperfusion-induced injury[J]. WORLD JOURNAL OF EMERGENCY MEDICINE,2022,13(1):46-53. |
| APA | Guo, Xiao-fang.,Gu, Shuang-shuang.,Wang, Jun.,Sun, Hao.,Zhang, Yu-juan.,...&Jiang, Lei.(2022).Protective effect of mesenchymal stem cell-derived exosomal treatment of hippocampal neurons against oxygen-glucose deprivation/reperfusion-induced injury.WORLD JOURNAL OF EMERGENCY MEDICINE,13(1),46-53. |
| MLA | Guo, Xiao-fang,et al."Protective effect of mesenchymal stem cell-derived exosomal treatment of hippocampal neurons against oxygen-glucose deprivation/reperfusion-induced injury".WORLD JOURNAL OF EMERGENCY MEDICINE 13.1(2022):46-53. |
入库方式： OAI收割
来源：上海药物研究所
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