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Quantitative imaging of natural products in fine brain regions using desorption electrospray ionization mass spectrometry imaging (DESI-MSI): Uncaria alkaloids as a case study

文献类型：期刊论文

作者Gao, Lei2,3; Zhang, Zijia3; Wu, Wenyong1,3; Deng, Yanping3; Zhi, Haijuan3; Long, Huali3; Lei, Min3; Hou, Jinjun3; Wu, Wanying2,3; Guo, De-an2,3
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
出版日期2022-05-31
页码9
ISSN号1618-2642
关键词Uncaria indole alkaloids DESI Quantitative mass spectrometry imaging Brain distribution Pineal gland On-tissue quantification
DOI10.1007/s00216-022-04130-3
通讯作者Hou, Jinjun(jinjun_hou@simm.ac.cn) ; Wu, Wanying(wanyingwu@simm.ac.cn)
英文摘要Uncaria species (Rubiaceae) are used as traditional Chinese medicines (TCMs) to treat central nervous system (CNS) diseases, and monoterpene indole alkaloids are the main bioactive constituents. Localization and quantification of CNS drugs in fine brain regions are important to provide insights into their pharmacodynamics, for which quantitative mass spectrometry imaging (MSI) has emerged as a powerful technique. A systematic study of the quantitative imaging of seven Uncaria alkaloids in rat brains using desorption electrospray ionization mass spectrometry imaging (DESI-MSI) was presented. The distribution of the alkaloids in thirteen brain regions was quantified successfully using the calibration curves generated by a modified on-tissue approach. The distribution trend of different Uncaria alkaloids in the rat brain was listed as monoterpene indole alkaloids > monoterpene oxindole alkaloids, R-configuration epimers >S-configuration epimers. Particularly, Uncaria alkaloids were detected directly in the pineal gland for the first time and their enrichment phenomenon in this region had an instructive significance in future pharmacodynamic studies.
WOS关键词PERFORMANCE LIQUID-CHROMATOGRAPHY ; RHYNCHOPHYLLINE ; RATS ; ISORHYNCHOPHYLLINE ; PHARMACOKINETICS ; METABOLITES ; INHIBITION ; SEROTONIN ; HOOKS ; MODEL
资助项目National Natural Science Foundation of China[82003915] ; National Natural Science Foundation of China[82003939]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
出版者SPRINGER HEIDELBERG
WOS记录号WOS:000803824900004
源URL[http://119.78.100.183/handle/2S10ELR8/301289]  
专题中国科学院上海药物研究所
通讯作者Hou, Jinjun; Wu, Wanying
作者单位1.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210029, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Gao, Lei,Zhang, Zijia,Wu, Wenyong,et al. Quantitative imaging of natural products in fine brain regions using desorption electrospray ionization mass spectrometry imaging (DESI-MSI): Uncaria alkaloids as a case study[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2022:9.
APA Gao, Lei.,Zhang, Zijia.,Wu, Wenyong.,Deng, Yanping.,Zhi, Haijuan.,...&Guo, De-an.(2022).Quantitative imaging of natural products in fine brain regions using desorption electrospray ionization mass spectrometry imaging (DESI-MSI): Uncaria alkaloids as a case study.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,9.
MLA Gao, Lei,et al."Quantitative imaging of natural products in fine brain regions using desorption electrospray ionization mass spectrometry imaging (DESI-MSI): Uncaria alkaloids as a case study".ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2022):9.

入库方式： OAI收割

来源：上海药物研究所

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