Integrated Chemoenzymatic Approach to Streamline the Assembly of Complex Glycopeptides in the Liquid Phase br
文献类型：期刊论文
| 作者 | Ma, Wenjing1,3; Deng, Yaqi3; Xu, Zhuojia1,3; Liu, Xingbang3; Chapla, Digantkumar G.2; Moremen, Kelley W.2; Wen, Liuqing3; Li, Tiehai1,3 |
| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| 出版日期 | 2022-05-25 |
| 卷号 | 144期号:20页码:9057-9065 |
| ISSN号 | 0002-7863 |
| DOI | 10.1021/jacs.2c01819 |
| 通讯作者 | Li, Tiehai(tiehaili@simm.ac.cn) |
| 英文摘要 | Glycosylation of proteins is a complicated post-translational modification. Despite the significant progress inglycoproteomics, accurate functions of glycoproteins are still ambiguous owing to the difficulty in obtaining homogeneousglycopeptides or glycoproteins. Here, we describe a streamlined chemoenzymatic method to prepare complex glycopeptides byintegrating hydrophobic tag-supported chemical synthesis and enzymatic glycosylations. The hydrophobic tag is utilized to facilitatepeptide chain elongation in the liquid phase and expeditious product separation. After removal of the tag, a series of glycans areinstalled on the peptides via efficient glycosyltransferase-catalyzed reactions. The general applicability and robustness of thisapproach are exemplified by efficient preparation of 16 well-defined SARS-CoV-2O-glycopeptides, 4 complex MUC1 glycopeptides,and a 31-mer glycosylated glucagon-like peptide-1. Our developed approach will open up a new range of easy access to variouscomplex glycopeptides of biological importance |
| WOS关键词 | SOLID-PHASE ; ENZYMATIC-SYNTHESIS ; PROTECTING-GROUP ; GLYCOSYLATION ; PEPTIDE ; MUC1 ; OLIGOSACCHARIDE ; EXPRESSION ; BASE ; TAG |
| 资助项目 | National Natural Science Foundation of China[22077130] ; Shanghai Municipal Science and Technology Major Project |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000805981500023 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/301313]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Li, Tiehai |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Univ Georgia, Complex Carbohydrate Res Ctr, Athens, GA 30602 USA 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Wenjing,Deng, Yaqi,Xu, Zhuojia,et al. Integrated Chemoenzymatic Approach to Streamline the Assembly of Complex Glycopeptides in the Liquid Phase br[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2022,144(20):9057-9065. |
| APA | Ma, Wenjing.,Deng, Yaqi.,Xu, Zhuojia.,Liu, Xingbang.,Chapla, Digantkumar G..,...&Li, Tiehai.(2022).Integrated Chemoenzymatic Approach to Streamline the Assembly of Complex Glycopeptides in the Liquid Phase br.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,144(20),9057-9065. |
| MLA | Ma, Wenjing,et al."Integrated Chemoenzymatic Approach to Streamline the Assembly of Complex Glycopeptides in the Liquid Phase br".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 144.20(2022):9057-9065. |
入库方式： OAI收割
来源：上海药物研究所
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