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Systematic Qualitative and Quantitative Analyses of Wenxin Granule via Ultra-High Performance Liquid Chromatography Coupled with Ion Mobility Quadrupole Time-of-Flight Mass Spectrometry and Triple Quadrupole-Linear Ion Trap Mass Spectrometry

文献类型：期刊论文

作者Mi, Yueguang3; Hu, Wandi3; Li, Weiwei3; Wan, Shiyu2; Xu, Xiaoyan3; Liu, Meiyu3; Wang, Hongda3; Mei, Quanxi2; Chen, Qinhua2; Yang, Yang2
刊名MOLECULES
出版日期2022-06-01
卷号27期号:11页码:21
关键词Wenxin granule multicomponent characterization HDMSE-HDDDA UHPLC-sMRM quality evaluation
DOI10.3390/molecules27113647
通讯作者Yang, Wenzhi(wzyang0504@tjutcm.edu.cn)
英文摘要Wenxin granule (WXG) is a popular traditional Chinese medicine (TCM) preparation for the treatment of arrhythmia disease. Potent analytical technologies are needed to elucidate its chemical composition and assess the quality differences among multibatch samples. In this work, both a multicomponent characterization and quantitative assay of WXG were conducted using two liquid chromatography-mass spectrometry (LC-MS) approaches. An ultra-high performance liquid chromatography-ion mobility quadrupole time-of-flight mass spectrometry (UHPLC/IM-QTOF-MS) approach combined with intelligent peak annotation workflows was developed to characterize the multicomponents of WXG. A hybrid scan approach enabling alternative data-independent and data-dependent acquisitions was established. We characterized 205 components, including 92 ginsenosides, 53 steroidal saponins, 14 alkaloids, and 46 others. Moreover, an optimized scheduled multiple reaction monitoring (sMRM) method was elaborated, targeting 24 compounds of WXG via ultra-high performance liquid chromatography-triple quadrupole linear ion trap mass spectrometry (UHPLC/QTrap-MS), which was validated based on its selectivity, precision, stability, repeatability, linearity, sensitivity, recovery, and matrix effect. By applying this method to 27 batches of WXG samples, the content variations of multiple markers from Notoginseng Radix et Rhizoma (21) and Codonopsis Radix (3) were depicted. Conclusively, we achieved the comprehensive multicomponent characterization and holistic quality assessment of WXG by targeting the non-volatile components.
WOS关键词SAPONINS ; POLYGONATUM ; MULTICOMPONENTS ; PHYTOCHEMISTRY ; CODONOPSIS ; DECOCTION ; RADIX ; KELI
资助项目National Natural Science Foundation of China[81872996] ; Natural Science Foundation of Tianjin of China[20JCYBJC00060] ; Sanming Project of Medicine in Shenzhen[SZZYSM202106004]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000809901700001
出版者MDPI
源URL[http://119.78.100.183/handle/2S10ELR8/301361]  
专题中国科学院上海药物研究所
通讯作者Yang, Wenzhi
作者单位1.Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, 501 Haike Rd, Shanghai 201203, Peoples R China
2.Shenzhen Baoan Authent TCM Therapy Hosp, Shenzhen 518101, Peoples R China
3.Tianjin Univ Tradit Chinese Med, State Key Lab Component Based Chinese Med, Tianjin Key Lab TCM Chem & Anal, 10 Poyanghu Rd, Tianjin 301617, Peoples R China
推荐引用方式
GB/T 7714		 Mi, Yueguang,Hu, Wandi,Li, Weiwei,et al. Systematic Qualitative and Quantitative Analyses of Wenxin Granule via Ultra-High Performance Liquid Chromatography Coupled with Ion Mobility Quadrupole Time-of-Flight Mass Spectrometry and Triple Quadrupole-Linear Ion Trap Mass Spectrometry[J]. MOLECULES,2022,27(11):21.
APA Mi, Yueguang.,Hu, Wandi.,Li, Weiwei.,Wan, Shiyu.,Xu, Xiaoyan.,...&Guo, Dean.(2022).Systematic Qualitative and Quantitative Analyses of Wenxin Granule via Ultra-High Performance Liquid Chromatography Coupled with Ion Mobility Quadrupole Time-of-Flight Mass Spectrometry and Triple Quadrupole-Linear Ion Trap Mass Spectrometry.MOLECULES,27(11),21.
MLA Mi, Yueguang,et al."Systematic Qualitative and Quantitative Analyses of Wenxin Granule via Ultra-High Performance Liquid Chromatography Coupled with Ion Mobility Quadrupole Time-of-Flight Mass Spectrometry and Triple Quadrupole-Linear Ion Trap Mass Spectrometry".MOLECULES 27.11(2022):21.

入库方式： OAI收割

来源：上海药物研究所

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