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A derivatization strategy for comprehensive identification of 2-and 3-hy-droxyl fatty acids by LC-MS

文献类型：期刊论文

作者Zhu, Zhancheng3,4,5,6; Li, Xiaomei3; Tang, Chunping3,4; Shen, Jianhua3; Liu, Jia1,2; Ye, Yang2,3,4,5,6
刊名ANALYTICA CHIMICA ACTA
出版日期2022-07-11
卷号1216页码:8
关键词Unsaturated 2 3-hydroxyl fatty acid ADMI derivatization Mcpba oxidation Stable isotope labeling Melanoma lung metastasis
ISSN号0003-2670
DOI10.1016/j.aca.2022.339981
通讯作者Liu, Jia(jia.liu@simm.ac.cn) ; Ye, Yang(yye@simm.ac.cn)
英文摘要2/3-Hydroxy fatty acids (2/3-OHFAs) are a class of important biological molecules closely related to many diseases, of which the unsaturated ones (2/3-OHUFAs) obtain special recognition for their bioactivities. However, the comprehensive identification of 2/3-OHFAs has been a daunting task due to the similarity of isomeric structures and lack of authentic standards. Herein, we report a strategy for the 2/3-OHUFA identification by using an in-house synthesized derivatization reagent, 4-amino-1,1-dimethylpiperidin-1-ium iodide hydrochloride (ADMI). Through ADMI derivatization, the diagnostic ion m/z 155.1 or 171.1 were produced by liquid chromatography-mass spectrometry (LC-MS) analysis, which could distinguish the 2-OH or 3-OH group, respectively. Then the meta-chloroperoxybenzoic acid (mcpba) was used to resolve the locations of double bonds in 2/3-OHUFAs by the characteristic cleavage of the newly formed epoxides. Thus, the identification of 2/3
WOS关键词ELECTRON-IMPACT EXCITATION ; DOUBLE-BOND POSITION ; PHOTODISSOCIATION MASS-SPECTROMETRY ; OZONE-INDUCED DISSOCIATION ; STRUCTURAL-CHARACTERIZATION ; OXIDATION ; ELUCIDATION ; ISOMERS ; LIPIDS ; IONS
资助项目National Natural Science Foundation of China[21920102003] ; Science and Technology Commission of Shanghai Municipality[20430780300] ; Key-Area Research and Development Program of Guangdong Province[2020B0303070002]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000808295500002
出版者ELSEVIER
源URL[http://119.78.100.183/handle/2S10ELR8/301367]  
专题新药研究国家重点实验室
通讯作者Liu, Jia; Ye, Yang
作者单位1.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310058, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Mat Med, Nat Prod Chem Dept, Shanghai 201203, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
6.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
推荐引用方式
GB/T 7714		 Zhu, Zhancheng,Li, Xiaomei,Tang, Chunping,et al. A derivatization strategy for comprehensive identification of 2-and 3-hy-droxyl fatty acids by LC-MS[J]. ANALYTICA CHIMICA ACTA,2022,1216:8.
APA Zhu, Zhancheng,Li, Xiaomei,Tang, Chunping,Shen, Jianhua,Liu, Jia,&Ye, Yang.(2022).A derivatization strategy for comprehensive identification of 2-and 3-hy-droxyl fatty acids by LC-MS.ANALYTICA CHIMICA ACTA,1216,8.
MLA Zhu, Zhancheng,et al."A derivatization strategy for comprehensive identification of 2-and 3-hy-droxyl fatty acids by LC-MS".ANALYTICA CHIMICA ACTA 1216(2022):8.

入库方式： OAI收割

来源：上海药物研究所

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