LC-MS/MS assay of fluoropezil and its two major metabolites in human plasma: an application to pharmacokinetic studies
文献类型：期刊论文
| 作者 | Guo, Runcong2,3; Hu, Jiafeng2,3; Jing, Jiao1; Liu, Ying3; Li, Jian3; Zhou, Yu3 ; Liu, Hong3; Zhou, Lei3; Chen, Xiaoyan2,3
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| 刊名 | BIOANALYSIS
 |
| 出版日期 | 2022-06-23 |
| 页码 | 13 |
| ISSN号 | 1757-6180 |
| 关键词 | assay bioanalysis DC20 fluoropezil LC-MS MS metabolites pharmacokinetics |
| DOI | 10.4155/bio-2022-0045 |
| 通讯作者 | Chen, Xiaoyan(xychen@simm.ac.cn) |
| 英文摘要 | Background: LC-MS/MS methods were developed for pharmacokinetic analysis and verified to measure fluoropezil, a new AchE inhibitor for Alzheimer's disease treatment, and its two primary metabolites (N-debenzyl fluoride fluoropezil [M1] and N-oxidized fluoropezil [M11]) in human plasma. Methods & results: Analytes were extracted from 50 mu l plasma using protein precipitation and separated by HPLC using a bridged ethyl hybrid column and gradient elution procedure. Analytical detection was performed with a triple quadrupole mass spectrometer and electrospray ionization source in multiple reaction monitoring mode. The LC-MS/MS method was fully validated. The quantification linear ranges were 0.100-50.0 ng/ml (fluoropezil), 0.0500-25.0 ng/ml (M1) and 0.0500-25.0 ng/ml (M11). Conclusion: A sensitive, reliable LC-MS/MS method was established and used successfully to explore the pharmacokinetics of fluoropezil. |
| WOS关键词 | DRUG ; INHIBITORS ; PROGRESS |
| 资助项目 | National Natural Science Foundation of China[82073924] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2021281] ; Jiangsu Kanion Pharmaceutical Co. Ltd (Jiangsu Lianyungang, China) ; Center for Drug Metabolism and Pharmacokinetics Research, Shanghai Institute of MateriaMedica, Chinese Academy of Sciences (Shanghai, China) |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| 出版者 | FUTURE SCI LTD |
| WOS记录号 | WOS:000814697800001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/301623]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Chen, Xiaoyan |
| 作者单位 | 1.Jiangsu Kan Pharmaceut Co Ltd, Jiangning Ind City Econ & Technol Dev Zone, Lianyungang 222001, Jiangsu, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, 501 Haike Rd & 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Runcong,Hu, Jiafeng,Jing, Jiao,et al. LC-MS/MS assay of fluoropezil and its two major metabolites in human plasma: an application to pharmacokinetic studies[J]. BIOANALYSIS,2022:13. |
| APA | Guo, Runcong.,Hu, Jiafeng.,Jing, Jiao.,Liu, Ying.,Li, Jian.,...&Chen, Xiaoyan.(2022).LC-MS/MS assay of fluoropezil and its two major metabolites in human plasma: an application to pharmacokinetic studies.BIOANALYSIS,13. |
| MLA | Guo, Runcong,et al."LC-MS/MS assay of fluoropezil and its two major metabolites in human plasma: an application to pharmacokinetic studies".BIOANALYSIS (2022):13. |
入库方式： OAI收割
来源：上海药物研究所
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