iTRAQ-based quantitative proteomics reveals biomarkers/pathways in psoriasis that can predict the efficacy of methotrexate
文献类型：期刊论文
| 作者 | Yan, K. X.4; Meng, Q.3; He, H.3; Zhu, H. W.3; Wang, Z. C.2; Han, L.4; Huang, Q.4; Zhang, Z. H.4; Yawalkar, N.1; Zhou, H.3
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| 刊名 | JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
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| 出版日期 | 2022-06-24 |
| 页码 | 12 |
| ISSN号 | 0926-9959 |
| DOI | 10.1111/jdv.18292 |
| 通讯作者 | Zhou, H.(zhouhu@simm.ac.cn) ; Xu, J. X.(jinhuaxu@fudan.edu.cn) |
| 英文摘要 | Background Methotrexate (MTX) is the first-line medicine to treat psoriasis. So far, there has been less research on protein biomarkers to predict its efficacy by the proteomic technique. Objectives To evaluate differentially expressed proteins in peripheral mononuclear cells (PBMCs) between good responders (GRs) and non-responders (NRs) after MTX treatment, compared with normal controls (NCs). Methods We quantified protein expression of PBMCs with four GRs and four NRs to MTX and four NCs by isobaric tags for relative and absolute quantification (iTRAQ), analysing and identifying proteins related to efficacy of MTX in 18 psoriatic patients. Results A total of 3177 proteins had quantitative information, and 403 differentially expressed proteins (fold change >= 1.2, P < 0.05) were identified. Compared to NCs, upregulated proteins (ANXA6, RPS27A, EZR, XRCC6), participating in the activation of NF-kappa B, the JAK-STAT pathway and neutrophil degranulation were detected in GRs. The proteins (GPV, FN1, STOM), involving platelet activation, signalling and aggregation as well as neutrophil degranulation were significantly downregulated in GRs. These proteins returned to normal levels after MTX treatment. Furthermore, Western blotting identified the expression of ANXA6 and STAT1 in PBMCs, which were significantly downregulated in GRs, but not in NRs. Conclusions We identified seven differentially expressed and regulated proteins (ANXA6, GPV, FN1, XRCC6, STOM, RPS27A and EZR) as biomarkers to predict MTX efficacy in NF-kappa B signalling, JAK-STAT pathways, neutrophil degranulation, platelet activation, signalling and aggregation. |
| WOS关键词 | PLATELET ACTIVATION ; HUMAN NEUTROPHILS ; ANNEXIN A6 ; FIBRONECTIN ; IDENTIFICATION ; ASSOCIATION ; EXPRESSION ; ARTHRITIS ; STOMATIN ; DOMAINS |
| WOS研究方向 | Dermatology |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000815116200001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/301635]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Zhou, H.; Xu, J. X. |
| 作者单位 | 1.Univ Bern, Bern Univ Hosp, Inselspital, Dept Dermatol, Bern, Switzerland 2.Fudan Univ, Huashan Hosp, Dept Clin Lab Med, Shanghai, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, CAS Key Lab Receptor Res, Shanghai, Peoples R China 4.Fudan Univ, Huashan Hosp, Inst Dermatol, Dept Dermatol, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, K. X.,Meng, Q.,He, H.,et al. iTRAQ-based quantitative proteomics reveals biomarkers/pathways in psoriasis that can predict the efficacy of methotrexate[J]. JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY,2022:12. |
| APA | Yan, K. X..,Meng, Q..,He, H..,Zhu, H. W..,Wang, Z. C..,...&Xu, J. X..(2022).iTRAQ-based quantitative proteomics reveals biomarkers/pathways in psoriasis that can predict the efficacy of methotrexate.JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY,12. |
| MLA | Yan, K. X.,et al."iTRAQ-based quantitative proteomics reveals biomarkers/pathways in psoriasis that can predict the efficacy of methotrexate".JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY (2022):12. |
入库方式： OAI收割
来源：上海药物研究所
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