Psoralen inhibits hepatitis B viral replication by down-regulating the host transcriptional machinery of viral promoters
文献类型：期刊论文
| 作者 | Ma, Xinna2,3; Li, Heng1,2; Gong, Ying1,2; Liu, Feifei2; Tong, Xiankun2 ; Zhu, Fenghua2; Yang, Xiaoqian2; Yang, Li2; Zuo, Jianping1,2,3
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| 刊名 | VIROLOGICA SINICA
 |
| 出版日期 | 2022-04-01 |
| 卷号 | 37期号:2页码:256-265 |
| 关键词 | Psoralen Hepatitis B virus (HBV) Forkhead box-O 1 (FOXO1) Transcription PPAR? co-activator 1? (PGC1?) |
| ISSN号 | 1674-0769 |
| DOI | 10.1016/j.virs.2022.01.027 |
| 通讯作者 | Yang, Li(yangli@simm.ac.cn) ; Zuo, Jianping(jpzuo@simm.ac.cn) |
| 英文摘要 | The hepatitis B virus (HBV) is a global public health challenge due to its highly contagious nature. It is estimated that almost 300 million people live with chronic HBV infection annually. Although nucleoside analogs markedly reduce the risk of liver disease progression, the analogs do not fully eradicate the virus. As such, new treatment options and drugs are urgently needed. Psoralen is a nourishing monomer of Chinese herb and is known to inhibit virus replication and inactivate viruses. In this study, we evaluated the potential of psoralen as an anti-HBV agent. Quantitative PCR and Southern blot analysis revealed that psoralen inhibited HBV replication in HepG2.2.15 cells in a concentration-dependent manner. Moreover, psoralen was also active against the 3TC/ETV-dual-resistant HBV mutant. Further investigations revealed that psoralen suppressed both HBV RNA transcription and core protein expression. The transcription factor FOXO1, a known target for PGC1?? co-activation, binds to HBV precore/core promoter enhancer II region and activates HBV RNA transcription. Co-immunoprecipitation showed that psoralen suppressed the expression of FOXO1, thereby decreasing the binding of FOXO1 co-activator PGC1?? to the HBV promoter. Overall, our results demonstrate that psoralen suppresses HBV RNA transcription by down regulating the expression of FOXO1 resulting in a reduction of HBV replication. |
| 资助项目 | National Science Fund[82104240] ; Shanghai Science and Technology Innovation Project[20S11906200] ; self-deployed scientific research projects (State Key Laboratory of Drug Research)[SIMM1903ZZ-03] |
| WOS研究方向 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000817760400012 |
| 出版者 | KEAI PUBLISHING LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/301744]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Yang, Li; Zuo, Jianping |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Lab Imniunophannacol, State Key Lab Drug Res, Shanghai 201203, Peoples R China 3.Shanghai Univ Tradit Chinese Med, Lab Immunol & Virol, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Xinna,Li, Heng,Gong, Ying,et al. Psoralen inhibits hepatitis B viral replication by down-regulating the host transcriptional machinery of viral promoters[J]. VIROLOGICA SINICA,2022,37(2):256-265. |
| APA | Ma, Xinna.,Li, Heng.,Gong, Ying.,Liu, Feifei.,Tong, Xiankun.,...&Zuo, Jianping.(2022).Psoralen inhibits hepatitis B viral replication by down-regulating the host transcriptional machinery of viral promoters.VIROLOGICA SINICA,37(2),256-265. |
| MLA | Ma, Xinna,et al."Psoralen inhibits hepatitis B viral replication by down-regulating the host transcriptional machinery of viral promoters".VIROLOGICA SINICA 37.2(2022):256-265. |
入库方式： OAI收割
来源：上海药物研究所
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