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Qualitative and quantitative determination of the primary active components and metabolites in human plasma after oral administration of Shuanghuanglian liquid

文献类型：期刊论文

作者Zhang, Shengman1,2; Liu, Ying2; Peng, Ni2; Chen, Xiaoyan1,2
刊名JOURNAL OF SEPARATION SCIENCE
出版日期2022-06-27
页码12
关键词baicalin chlorogenic acid forsythin liquid chromatography-tandem mass spectrometry Shuanghuanglian liquid
ISSN号1615-9306
DOI10.1002/jssc.202200121
通讯作者Chen, Xiaoyan(xychen@simm.ac.cn)
英文摘要Shuanghuanglian oral liquid is a common traditional Chinese medicine used to treat respiratory tract infections. Its major components are baicalin, chlorogenic acid, and forsythin. In this study, the main drug-related components in human plasma after oral administration of Shuanghuanglian were initially identified using ultra-performance liquid chromatography-ultraviolet detector/quadrupole time-of-flight mass spectrometry. Thirteen components from baicalin were identified, including the parent drug baicalin and aglycone baicalein. Only one metabolite related to chlorogenic acid, a sulfate conjugate formed after hydrolysis, and one metabolite related to forsythin, a sulfate conjugate of forsythin aglycone, were detected. Subsequently, a liquid chromatography-tandem mass spectrometry method was established and validated to simultaneously determine baicalin and baicalein, the primary active components. After simple protein precipitation, the analytes were separated on a BEH C18 column using a 5 min-gradient elution to avoid interference from baicalin isomers and their in-source dissociation. Excellent linearity was observed over the concentration ranges of 5.00-2000 ng/ml for baicalin and 1.00-100 ng/ml for baicalein. The validated method was successfully applied to a pharmacokinetic study of an oral administration of 60 ml Shuanghuanglian in healthy subjects. This study provided a foundation to investigate the clinical efficacy and safety of Shuanghuanglian further.
WOS关键词TANDEM MASS-SPECTROMETRY ; RAT PLASMA ; BAICALIN ; CHROMATOGRAPHY ; WOGONIN ; OROXYLIN ; WOGONOSIDE ; RADIX
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000816843200001
出版者WILEY-V C H VERLAG GMBH
源URL[http://119.78.100.183/handle/2S10ELR8/301800]  
专题中国科学院上海药物研究所
通讯作者Chen, Xiaoyan
作者单位1.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, 501 Haike Rd, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Shengman,Liu, Ying,Peng, Ni,et al. Qualitative and quantitative determination of the primary active components and metabolites in human plasma after oral administration of Shuanghuanglian liquid[J]. JOURNAL OF SEPARATION SCIENCE,2022:12.
APA Zhang, Shengman,Liu, Ying,Peng, Ni,&Chen, Xiaoyan.(2022).Qualitative and quantitative determination of the primary active components and metabolites in human plasma after oral administration of Shuanghuanglian liquid.JOURNAL OF SEPARATION SCIENCE,12.
MLA Zhang, Shengman,et al."Qualitative and quantitative determination of the primary active components and metabolites in human plasma after oral administration of Shuanghuanglian liquid".JOURNAL OF SEPARATION SCIENCE (2022):12.

入库方式： OAI收割

来源：上海药物研究所

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