Comparative study of the immunomodulatory effects of different fucoidans from Saccharina japonica mediated by scavenger receptors on RAW 264.7 macrophages
文献类型：期刊论文
| 作者 | Cui, Meiyu2,3,4 ; Li, Xiaohong1; Geng, Lihua3,4; Wu, Ning3,4; Wang, Jing3,4; Deng, Zhenzhen2,3,4; Li, Zhi2,3,4; Zhang, Quanbin3,4
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| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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| 出版日期 | 2022-08-31 |
| 卷号 | 215页码:253-261 |
| 关键词 | Fucoidan Saccharina japonica Scavenger receptor Macrophage SR-A CD36 LOX-1 Immunomodulatory |
| ISSN号 | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2022.06.111 |
| 通讯作者 | Geng, Lihua(lhgeng@qdio.ac.cn) |
| 英文摘要 | Scavenger receptors (SRs) have been shown to participate in regulating the immune response of macrophages, and fucoidan from Fucus vesiculosus has been verified as a ligand of class A SRs (SR-A). However, the roles of SRs in the immunomodulatory activity of fucoidan from Saccharina japonica are not clear. Thus, we performed a comparative study of the immunomodulatory activities of six different fucoidans from S. japonica on RAW 264.7 macrophages, and the roles of SRs in the processes were studied. Six fucoidans (0.5 M FPS, 1 M FPS, 2 M FPS, 0.5 M DFPS, 1 M DFPS and 2 M FPS) had different molecular weights and chemical compositions. Griess reagent system, ELISA and RT-qPCR results showed that different fucoidans displayed different stimulation of macro-phages to secrete NO, IL-6, IL-1 beta and TNF-alpha, as well as differences in the upregulation of their gene expressiones. Flow cytometric analysis of the protein expression level indicated the upregulation of TLR4 after treatment with all the fucoidans but different expressions of SRs. Furthermore, only 0.5 M DFPS and 1 M DFPS were confirmed to be ligands of SR-A through the competitive binding assay with Ac-LDL bound to the fluorescent probe DiI by flow cytometry. Our results revealed that fucoidans with low molecular weight and heterogeneity more easily bound to SRs and contributed to their immunomodulatory effects. This comparative study might promote the biological study of targeted SRs and the discovery of new pharmacological mechanisms of different fucoidans. |
| 资助项目 | Shandong Provincial Natural Science Foundation[ZR2019BD053] ; China Postdoctoral Science Founda-tion[2018M642710] ; Shandong Province Major Science and Technology Innovation Project[2019JZZY010818] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000818510600008 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/179664]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Geng, Lihua |
| 作者单位 | 1.Qingdao Municipal Hosp, Dept endocrinol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Lab Marine Biol & Biotechnol, Qingdao Natl Lab Marine Sci & Technol, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, Meiyu,Li, Xiaohong,Geng, Lihua,et al. Comparative study of the immunomodulatory effects of different fucoidans from Saccharina japonica mediated by scavenger receptors on RAW 264.7 macrophages[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2022,215:253-261. |
| APA | Cui, Meiyu.,Li, Xiaohong.,Geng, Lihua.,Wu, Ning.,Wang, Jing.,...&Zhang, Quanbin.(2022).Comparative study of the immunomodulatory effects of different fucoidans from Saccharina japonica mediated by scavenger receptors on RAW 264.7 macrophages.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,215,253-261. |
| MLA | Cui, Meiyu,et al."Comparative study of the immunomodulatory effects of different fucoidans from Saccharina japonica mediated by scavenger receptors on RAW 264.7 macrophages".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 215(2022):253-261. |
入库方式： OAI收割
来源：海洋研究所
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