Establishment and preliminary study of electrophysiological techniques in a typical red tide species
文献类型：期刊论文
| 作者 | Wang, Zhongshi3,4,5,6; Yu, Zhiming1,3,4,5,6 ; He, Liyan3,5,6; Zhu, Jianan3,5,6; Liu, Lidong2; Song, Xiuxian3,4,5,6
|
| 刊名 | SCIENCE OF THE TOTAL ENVIRONMENT
 |
| 出版日期 | 2022-09-20 |
| 卷号 | 840页码:11 |
| ISSN号 | 0048-9697 |
| 关键词 | Electrophysiological technique Dinoflagellate Protoplast Voltage-clamp Alexandrium pacificum |
| DOI | 10.1016/j.scitotenv.2022.156698 |
| 通讯作者 | Yu, Zhiming(zyu@qdio.ac.cn) |
| 英文摘要 | Electrophysiology studies the electrical properties of cells and tissues including bioelectrical signals and membrane ion channel activities. As an important means to reveal ion channel related physiological functions and the underlying mechanisms, electrophysiological techniques have been widely used in studies of animals, higher plants and algae that are closely related to higher plants. However, few electrophysiological studies have been carried out in red tide organisms, especially in dinoflagellates, which is mainly due to the complex surface structure of dinoflagellate amphiesma. In this study, the surface amphiesma of Alexandrium pacificum, a typical red tide species, was removed by centrifugation, low-temperature treatment and enzymatic treatment. In all three treatments, low-temperature treatment with 4 C for 2 h had high ecdysis rate and high fixation rate, and the treated cells were easy to puncture, so low temperature treatment was used as a preprocessing treatment for subsequent current recording. Acquired protoplasts of A. pacificum were identified by calcofluor fluorescence and immobilized by poly-lysine. A modified "puncture " single-electrode voltage-clamp recording was first applied to dinoflagellates, and voltage-gated currents, which had the characteristics of outward K+ current and inward Cl(- )current, were recorded and confirmed by ion replacement, indicating the voltage-gated currents were mixed. This method can be used as a technical basis for the electrophysiological study of dinoflagellates and provides a new perspective for the study of stress tolerance, red tide succession, and the regulation of physiological function of dinoflagellates. |
| 资助项目 | Institute of Oceanology, Chinese Academy of Sciences ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2021QNLM040001-1] ; Taishan Program of Shandong Province of 2019 ; National Natural Science Foundation of China[42006120] ; Special Research Assistant Program of Chinese Academy of Sciences |
| WOS研究方向 | Environmental Sciences & Ecology |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000816997600005 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/179634]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Yu, Zhiming |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Univ British Columbia, Djavad Mowafaghian Ctr Brian Hlth, Dept Med, Vancouver, BC, Canada 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Zhongshi,Yu, Zhiming,He, Liyan,et al. Establishment and preliminary study of electrophysiological techniques in a typical red tide species[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2022,840:11. |
| APA | Wang, Zhongshi,Yu, Zhiming,He, Liyan,Zhu, Jianan,Liu, Lidong,&Song, Xiuxian.(2022).Establishment and preliminary study of electrophysiological techniques in a typical red tide species.SCIENCE OF THE TOTAL ENVIRONMENT,840,11. |
| MLA | Wang, Zhongshi,et al."Establishment and preliminary study of electrophysiological techniques in a typical red tide species".SCIENCE OF THE TOTAL ENVIRONMENT 840(2022):11. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。