中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 重庆绿色智能技术研究院
  3. 中国科学院重庆绿色智能技术研究院
Highly Sensitive Fluorescence Assay for miRNA Detection: Investigation of the DNA Spacer Effect on the DSN Enzyme Activity toward Magnetic-Bead-Tethered Probes

文献类型：期刊论文

作者Djebbi, Khouloud2,3,4; Shi, Biao2,3; Weng, Ting2,3; Bahri, Mohamed2,3,4; Elaguech, Mohamed Amin2,3,4; Liu, Jin1,3; Tlili, Chaker2,3; Wang, Deqiang2,3,4
刊名ACS OMEGA
出版日期2022-01-18
卷号7期号:2页码:2224-2233
ISSN号2470-1343
DOI10.1021/acsomega.1c05775
通讯作者Liu, Jin(18983079956@189.cn) ; Tlili, Chaker(chakertlili@cigit.ac.cn) ; Wang, Deqiang(dqwang@cigit.ac.cn)
英文摘要Researchers have recently designed various biosensors combining magnetic beads (MBs) and duplex-specific nuclease (DSN) enzyme to detect miRNAs. Yet, the interfacial mechanisms for surface-based hybridization and DSN-assisted target recycling are relatively not well understood. Thus, herein, we developed a highly sensitive and selective fluorescent biosensor to study the phenomenon that occurs on the local microenvironment surrounding the MB-tethered DNA probe via detecting microRNA-21 as a model. Using the above strategy, we investigated the influence of different DNA spacers, base-pair orientations, and surface densities on DSN-assisted target recycling. As a result, we were able to detect as low as 170 aM of miR-21 under the optimized conditions. Moreover, this approach exhibits a high selectivity in a fully matched target compared to a single-base mismatch, allowing the detection of miRNAs in serum with improved recovery. These results are attributed to the synergetic effect between the DSN enzyme activity and the neutral DNA spacer (triethylene glycol: TEG) to improve the miRNA detections sensitivity. Finally, our strategy could create new paths for detecting microRNAs since it obliterates the enzyme-mediated cascade reaction used in previous studies, which is more expensive, more time-consuming, less sensitive, and requires double catalytic reactions.
资助项目National Natural Science Foundation of China[31800711] ; Natural Science Foundation of Chongqing, China[cstc2020jcyj-msxmX1007] ; Natural Science Foundation of Chongqing, China[cstc2018jcyjAX030] ; Natural Science Foundation of Chongqing, China[cstc2018jcyjAX0308]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000772025700063
出版者AMER CHEMICAL SOC
源URL[http://119.78.100.138/handle/2HOD01W0/15080]  
专题中国科学院重庆绿色智能技术研究院
通讯作者Liu, Jin; Tlili, Chaker; Wang, Deqiang
作者单位1.Univ Chinese Acad Sci UCAS, Chongqing Gen Hosp, Dept Pathol, Chongqing 400013, Peoples R China
2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China
3.Univ Chinese Acad Sci UCAS, Chongqing Sch, Chongqing 400714, Peoples R China
4.Univ Chinese Acad Sci UCAS, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Djebbi, Khouloud,Shi, Biao,Weng, Ting,et al. Highly Sensitive Fluorescence Assay for miRNA Detection: Investigation of the DNA Spacer Effect on the DSN Enzyme Activity toward Magnetic-Bead-Tethered Probes[J]. ACS OMEGA,2022,7(2):2224-2233.
APA Djebbi, Khouloud.,Shi, Biao.,Weng, Ting.,Bahri, Mohamed.,Elaguech, Mohamed Amin.,...&Wang, Deqiang.(2022).Highly Sensitive Fluorescence Assay for miRNA Detection: Investigation of the DNA Spacer Effect on the DSN Enzyme Activity toward Magnetic-Bead-Tethered Probes.ACS OMEGA,7(2),2224-2233.
MLA Djebbi, Khouloud,et al."Highly Sensitive Fluorescence Assay for miRNA Detection: Investigation of the DNA Spacer Effect on the DSN Enzyme Activity toward Magnetic-Bead-Tethered Probes".ACS OMEGA 7.2(2022):2224-2233.

入库方式： OAI收割

来源：重庆绿色智能技术研究院

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。