Single-Molecule Identification of the Conformations of Human C-Reactive Protein and Its Aptamer Complex with Solid-State Nanopores
文献类型：期刊论文
| 作者 | Wu, Ji1,2; Liang, Liyuan1,2 ; Zhang, Mingkun1,2; Zhu, Rui1,2; Wang, Zhong1,2; Yin, Yajie1,2; Yin, Bohua1,2; Weng, Ting1,2; Fang, Shaoxi1,2; Xie, Wanyi1,2
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| 刊名 | ACS APPLIED MATERIALS & INTERFACES
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| 出版日期 | 2022-03-16 |
| 卷号 | 14期号:10页码:12077-12088 |
| 关键词 | solid-state nanopore C-reactive protein aptamer single-molecule conformation |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.2c00453 |
| 通讯作者 | Liang, Liyuan(liangliyuan@cigit.ac.cn) |
| 英文摘要 | Human C-reactive protein (CRP) is an established inflammatory biomarker and was proved to be potentially relevant to disease pathology and cancer progression. A large body of methodologies have been reported for CRP analysis, including electrochemical/optical biosensors, aptamer, or antibody-based detection. Although the detection limit is rather low until pg/uL, most of which are time-consuming and relatively expensive, and few of them provided CRP single-molecule information. This work demonstrated the nanopore-based approach for the characterization of CRP conformation under versatile conditions. With an optimized pore of 14 nm in diameter, we achieved the detection limit as low as 0.3 ng/mu L, voltage polarity significantly influences the electro-osmotic force and CRP translocation behavior, and the pentameric conformation of CRP may dissociate into pro-inflammatory CRP isoforms and monomeric CRP at bias potential above 300 mV. CRP tends to translocate through nanopores faster along with the increase in pH values, due to more surface charge on both CRP and pore inner wall and stronger electro-osmotic force. The CRP could specifically bind with its aptamer of different concentrations to form complexes, and the complexes exhibited distinguishable nanopore translocation behavior compared with CRP alone. The variation of the molar ratio of aptamer significantly influences the orientation of CRP translocation. The plasma test under physiological conditions displayed the ability of the nanopore system on the CRP identification with a concentration of 3 ng/mu L. |
| 资助项目 | Excellent Youth Foundation of Chongqing Scientific Committee[cstc2021jcyj-jqX0030] ; West Light Foundation of the Chinese Academy of Sciences ; Natural Science Foundation of Beibei, Chongqing, China[E1316101] ; National Natural Science Foundation of China[31800711] ; Natural Science Foundation of Chongqing, China[cstc2020jcyj-msxmX1007] ; Natural Science Foundation of Chongqing, China[cstc2018jcyjAX0304] ; Natural Science Foundation of Chongqing, China[cstc2018jcyjAX0308] ; Exceptional Young Talents Project[cstc2021ycjh-bgzxm0016] ; University of the Chinese Academy of Sciences |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000787549000006 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/15894]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Liang, Liyuan |
| 作者单位 | 1.Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400714, Peoples R China 2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing Key Lab Multiscale Mfg Technol, Chongqing 400714, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Ji,Liang, Liyuan,Zhang, Mingkun,et al. Single-Molecule Identification of the Conformations of Human C-Reactive Protein and Its Aptamer Complex with Solid-State Nanopores[J]. ACS APPLIED MATERIALS & INTERFACES,2022,14(10):12077-12088. |
| APA | Wu, Ji.,Liang, Liyuan.,Zhang, Mingkun.,Zhu, Rui.,Wang, Zhong.,...&Wang, Deqiang.(2022).Single-Molecule Identification of the Conformations of Human C-Reactive Protein and Its Aptamer Complex with Solid-State Nanopores.ACS APPLIED MATERIALS & INTERFACES,14(10),12077-12088. |
| MLA | Wu, Ji,et al."Single-Molecule Identification of the Conformations of Human C-Reactive Protein and Its Aptamer Complex with Solid-State Nanopores".ACS APPLIED MATERIALS & INTERFACES 14.10(2022):12077-12088. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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