Trapping and releasing of single microparticles and cells in a microfluidic chip
文献类型：期刊论文
| 作者 | Lv, Dan1; Zhang, Xiaoling2,3; Xu, Mengli1; Cao, Wenyue1; Liu, Xing1; Deng, Jinan1; Yang, Jun1 ; Hu, Ning1
|
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2022-07-03 |
| 页码 | 10 |
| ISSN号 | 0173-0835 |
| 关键词 | hydrodynamic trapping negative dielectrophoresis particle manipulation single-cell analysis single-cell releasing |
| DOI | 10.1002/elps.202200091 |
| 通讯作者 | Hu, Ning(huning@cqu.edu.cn) |
| 英文摘要 | A microfluidic device was designed and fabricated to capture single microparticles and cells by using hydrodynamic force and selectively release the microparticles and cells of interest via negative dielectrophoresis by activating selected individual microelectrodes. The trap microstructure was optimized based on numerical simulation of the electric field as well as the flow field. The capture and selective release functions of the device were verified by multi-types microparticles with different diameters and K562 cells. The capture efficiencies/release efficiencies were 95.55% +/- 0.43%/96.41% +/- 1.08% and 91.34% +/- 0.01%/93.67% +/- 0.36% for microparticles and cells, respectively. By including more traps and microelectrodes, the device can achieve high throughput and realize the visual separation of microparticles/cells of interest in a large number of particle/cell groups. |
| 资助项目 | National Natural Science Foundation of China[32071408] ; National Natural Science Foundation of China[21827812] ; National Natural Science Foundation of China[81871450] ; National Natural Science Foundation of China[32101114] ; Natural Science Foundation of Chongqing[cstc2018jcyjAX0389] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000820116500001 |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/15946]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Hu, Ning |
| 作者单位 | 1.Chongqing Univ, Bioengn Coll, Key Lab Biorheol Sci & Technol, Minist Educ, Chongqing 400030, Peoples R China 2.Univ Chinese Acad Sci, Chongqing Sch, Chongqing, Peoples R China 3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lv, Dan,Zhang, Xiaoling,Xu, Mengli,et al. Trapping and releasing of single microparticles and cells in a microfluidic chip[J]. ELECTROPHORESIS,2022:10. |
| APA | Lv, Dan.,Zhang, Xiaoling.,Xu, Mengli.,Cao, Wenyue.,Liu, Xing.,...&Hu, Ning.(2022).Trapping and releasing of single microparticles and cells in a microfluidic chip.ELECTROPHORESIS,10. |
| MLA | Lv, Dan,et al."Trapping and releasing of single microparticles and cells in a microfluidic chip".ELECTROPHORESIS (2022):10. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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