Janus wireframe DNA cube-based 3D nanomachine for rapid and stable fluorescence detection of exosomal microRNA
文献类型：期刊论文
| 作者 | Xu, Yuan1,2,3,4; Li, Xinmin5,6; Niu, Changchun3; Wu, Haiping5; Yong, Yutao1,3; Qi, Caihong3; Gong, Wei5; Bai, Huijie5; Chen, Yirong5; Ding, Shijia5 |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2022-09-15 |
| 卷号 | 212页码:8 |
| 关键词 | Janus wireframe DNA cube Catalytic hairpin assembly DNA nanomachine Exosomal miRNA |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2022.114405 |
| 通讯作者 | Liao, Pu(liaopu@ucas.ac.cn) |
| 英文摘要 | Exosomal microRNAs (miRNAs) have been demonstrated to be credible biomarkers for the diagnosis and monitoring of tumors. Nevertheless, developing simple, rapid, and stable biosensing strategies that are capable of accurately detecting exosomal miRNAs remains a challenge. Herein, an accelerated and biostable threedimensional (3D) nanomachine based on Janus wireframe DNA cube was constructed for sensitive fluorescence measurement of exosomal miRNA. The Janus wireframe DNA cube could propel target exosomal miRNA21 rapid movement on its surface by catalytic hairpin assembly (CHA), releasing a massive fluorescence signal. Benefiting from the Janus wireframe DNA cube, the developed 3D nanomachine exhibited significantly improved reaction rate and enhanced biostability in complex biofluids compared to conventional CHA. As a result, this fluorescence biosensing strategy achieved rapid, stable, and single-step detection of exosomal miRNA-21 with the detection limit down to the picomole level. Therefore, this work offers a brief sensing tool for nucleic acid biomarkers detection, which has great application potential in tumor diagnosis. |
| 资助项目 | Technology Innovation and Application Development Special Project of Chongqing, China[cstc2020jscx-sbqwX0011] ; Natural Science Foundation of Chongqing, China[cstc2019jcyj-msxmX0276] |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000806013300003 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/15958]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Liao, Pu |
| 作者单位 | 1.Chongqing Med Univ, Chongqing 400016, Peoples R China 2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China 3.Chongqing Gen Hosp, Dept Clin Lab, Chongqing 400016, Peoples R China 4.North Sichuan Med Coll, Dept Clin Lab, Affiliated Hosp, Nanchong 637000, Peoples R China 5.Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China 6.Chongqing Hosp Tradit Chinese Med, Dept Lab Med, Chongqing 400016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Yuan,Li, Xinmin,Niu, Changchun,et al. Janus wireframe DNA cube-based 3D nanomachine for rapid and stable fluorescence detection of exosomal microRNA[J]. BIOSENSORS & BIOELECTRONICS,2022,212:8. |
| APA | Xu, Yuan.,Li, Xinmin.,Niu, Changchun.,Wu, Haiping.,Yong, Yutao.,...&Liao, Pu.(2022).Janus wireframe DNA cube-based 3D nanomachine for rapid and stable fluorescence detection of exosomal microRNA.BIOSENSORS & BIOELECTRONICS,212,8. |
| MLA | Xu, Yuan,et al."Janus wireframe DNA cube-based 3D nanomachine for rapid and stable fluorescence detection of exosomal microRNA".BIOSENSORS & BIOELECTRONICS 212(2022):8. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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