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Highly sensitive fluorescence multiplexed miRNAs biosensors for accurate clinically diagnosis lung cancer disease using LNA-modified DNA probe and DSN enzyme

文献类型：期刊论文

作者Djebbi, Khouloud1,2,3; Xing, Juanxia4,5; Weng, Ting1,2; Bahri, Mohamed1,2,3; Elaguech, Mohamed Amin1,2,3; Du, Chao4,5; Shi, Biao1,2; Hu, Li4; He, Shixuan1,2; Liao, Pu2,4,5
刊名ANALYTICA CHIMICA ACTA
出版日期2022-05-22
卷号1208页码:9
ISSN号0003-2670
关键词Lung cancer microRNAs Fluorescence assay Multiplexed DSN Magnetic beads
DOI10.1016/j.aca.2022.339778
通讯作者Liao, Pu() ; Tlili, Chaker(chakertlili@cigit.ac.cn) ; Wang, Deqiang()
英文摘要With the emergence of microRNAs as key biomarkers for disease diagnosis such as lung cancer, various techniques have been settled for their detection. However, these current methods require different amplification steps since numerous challenges for detecting circulating miRNAs are attributable to their intrinsic properties accounting for tiny sizes, high sequence similarity, and low abundance. Duplex specific nuclease (DSN)-based microRNA amplification has recently gained interest in biosensing applications thanks to its catalytic activity based on target recycling. In this context, we designed a highly selective, sensitive, and multiplexed fluorescence-based biosensor combining DSN enzyme and magnetic & nbsp;beads to detect three distinct microRNAs, including microRNA-21, microRNA-210, and microRNA-486-5p. By exploiting the above approach, we were able to detect as low as 98 aM, 120 aM, and 300 aM of mir-21, miR-210, and miR-486-5p, respectively. Furthermore, this recommended strategy displays a high selectivity toward an entirely matched target than the off-target. These results are ascribed to the potent DSN enzyme activity and to the locked nucleic acid (LNA)-modified DNA probe that boosted the heteroduplex probe/target stability. Lastly, our proposed method was applied to detect microRNAs in the serum samples and displayed a high efficacy to discriminate between healthy controls and lung cancer patients. Furthermore, the analytical accuracy of the proposed strategy was validated with the computed tomography (CT) technique of the chest. Thus based on these findings, this strategy could open new directions for detecting microRNAs associated with several diseases. (c) 2022 Elsevier B.V. All rights reserved.
WOS研究方向Chemistry
语种英语
出版者ELSEVIER
WOS记录号WOS:000804193000011
源URL[http://119.78.100.138/handle/2HOD01W0/16196]  
专题中国科学院重庆绿色智能技术研究院
通讯作者Liao, Pu; Tlili, Chaker; Wang, Deqiang
作者单位1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China
2.Univ Chinese Acad Sci UCAS, Chongqing Sch, Chongqing 400714, Peoples R China
3.Univ Chinese Acad Sci UCAS, Beijing 100049, Peoples R China
4.Chongqing Gen Hosp, Dept Lab Med, Chongqing 400014, Peoples R China
5.Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China
推荐引用方式
GB/T 7714		 Djebbi, Khouloud,Xing, Juanxia,Weng, Ting,et al. Highly sensitive fluorescence multiplexed miRNAs biosensors for accurate clinically diagnosis lung cancer disease using LNA-modified DNA probe and DSN enzyme[J]. ANALYTICA CHIMICA ACTA,2022,1208:9.
APA Djebbi, Khouloud.,Xing, Juanxia.,Weng, Ting.,Bahri, Mohamed.,Elaguech, Mohamed Amin.,...&Wang, Deqiang.(2022).Highly sensitive fluorescence multiplexed miRNAs biosensors for accurate clinically diagnosis lung cancer disease using LNA-modified DNA probe and DSN enzyme.ANALYTICA CHIMICA ACTA,1208,9.
MLA Djebbi, Khouloud,et al."Highly sensitive fluorescence multiplexed miRNAs biosensors for accurate clinically diagnosis lung cancer disease using LNA-modified DNA probe and DSN enzyme".ANALYTICA CHIMICA ACTA 1208(2022):9.

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来源：重庆绿色智能技术研究院

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