In vivo toxicity assessment of four types of graphene quantum dots (GQDs) using mRNA sequencing
文献类型：期刊论文
| 作者 | Deng, Shun1; Zhang, Enming2; Wang, Yan3; Zhao, Yunyang4; Yang, Zezhong5; Zheng, Bingxin1; Mu, Xiaoyuan1; Deng, Xuangen1; Shen, Hai1; Rong, Haibo1 |
| 刊名 | TOXICOLOGY LETTERS
 |
| 出版日期 | 2022-06-15 |
| 卷号 | 363页码:55-66 |
| 关键词 | Graphene quantum dots Toxicity Zebrafish Transcriptomic responses |
| ISSN号 | 0378-4274 |
| DOI | 10.1016/j.toxlet.2022.05.006 |
| 通讯作者 | Deng, Shun(dshun1979@swu.edu.cn) |
| 英文摘要 | GQDs show great potential in drug carriers, bioimaging, biosensors, theranostics, and are recently reported as promising therapeutic agents to treat amyloid-related diseases such as Parkinson's disease and inflammations such as colitis. However, current toxicity data about GQDs based on in vivo toxicity assessments remain scarce. In the study, we examined the mRNA expression changes of zebrafish embryos exposed to four types of GQDs, including raw graphene quantum dots (R-GQDs), graphene oxide quantum dots (GOQDs), carboxyl GQDs (CGQDs), and aminated GQDs (A-GQDs). Firstly, we treated embryos with the four GQDs at three concentrations (50, 100, and 200 mu g/mL), and found that only A-GQDs caused embryonic developmental arrest at 100 and 200 mu g/mL with significantly decreased survival rates and heartbeat rates, as well as the elevated malformation rates. Next, we analyzed the mRNA sequencing data acquired from zebrafish embryos exposed to the four GQDs for 7 days at 100 mu g/mL, and found that all GQDs can act on potassium (K+) and calcium (Ca2+) channels, and spliceosomes with varying degrees of regulatory effects. Compared to other GQDs, A-GQDs can strongly perturb the anticoagulant protein C (PC) pathway via activating most genes associated with complement and coagulation system, cell adhesion molecules (CAMs), and MAPK. In conclusion, this study provided substantial transcriptomic data underlying the common signaling pathways induced by various types of GQDs and pointed out the specific toxicity of A-GQDs on hemostatic system. |
| 资助项目 | Central Government Funds of Guiding Local Scientific and Technological Development for Sichuan Province[2021ZYD0068] ; Sichuan Medical Association[S20022] ; Fundamental Research Fund for the Central Universities from Ministry of Education, PRC[2015CP001] |
| WOS研究方向 | Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000808581100001 |
| 出版者 | ELSEVIER IRELAND LTD |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/16202]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Deng, Shun |
| 作者单位 | 1.Sichuan Prov Orthoped Hosp, Chengdu 610041, Peoples R China 2.Beijing Sport Univ, Sch Sports Med & Phys Therapy, Beijing 100084, Peoples R China 3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China 4.Wenzhou Med Univ, Sci Res Ctr, Wenzhou 325035, Peoples R China 5.Tianjin Acad Agr Sci, Inst Plant Protect, Tianjin 300192, Peoples R China |
| 推荐引用方式 GB/T 7714 | Deng, Shun,Zhang, Enming,Wang, Yan,et al. In vivo toxicity assessment of four types of graphene quantum dots (GQDs) using mRNA sequencing[J]. TOXICOLOGY LETTERS,2022,363:55-66. |
| APA | Deng, Shun.,Zhang, Enming.,Wang, Yan.,Zhao, Yunyang.,Yang, Zezhong.,...&Pei, Desheng.(2022).In vivo toxicity assessment of four types of graphene quantum dots (GQDs) using mRNA sequencing.TOXICOLOGY LETTERS,363,55-66. |
| MLA | Deng, Shun,et al."In vivo toxicity assessment of four types of graphene quantum dots (GQDs) using mRNA sequencing".TOXICOLOGY LETTERS 363(2022):55-66. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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