Genetic Engineering of Adipose-Derived Stem Cells Using Biodegradable and Lipid-Like Highly Branched Poly(beta-amino ester)s
文献类型：期刊论文
| 作者 | Li, Qiuxia1; Sun, Litao1; Huang, Xiaobei2; Liu, Shuai3; Yong, Haiyang1; Wang, Chenfei1; Li, Jianzhong4; Zhou, Dezhong1 |
| 刊名 | ACS MACRO LETTERS
 |
| 出版日期 | 2022-05-17 |
| 卷号 | 11期号:5页码:636-642 |
| DOI | 10.1021/acsmacrolett.2c00095 |
| 通讯作者 | Liu, Shuai(shuailiu@zju.edu.cn) ; Li, Jianzhong(jianzhong-0520@163.com) ; Zhou, Dezhong(dezhong.zhou@xjtu.edu.cn) |
| 英文摘要 | Biodegradable and lipid-like highly branched poly(beta-amino ester)s, HPAESA, were developed to enhance the biological functions of adipose-derived stem cells by gene transfection. Biodegradability reduces the cytotoxicity of HPAESA and enables controlled DNA release. Lipid mimicry enhances cellular uptake and endosomal escape of HPAESA/DNA polyplexes. HPAESA are able to transfect rat adipose-derived stem cells (rADSs) and human ADSCs (hADSCs) with orders of magnitude higher efficiency than commercial gene transfection reagents, with cell viability exceeding 90%. Most importantly, HPAESA can effectively transfer the nerve growth factor (NGF)-encoding plasmid to rADSCs and induce high NGF secretion, which significantly promotes neurite outgrowth of PC12 cells. |
| 资助项目 | National Natural Science Foundation of China (NSFC)[51903202] ; National Natural Science Foundation of China (NSFC)[81903268] ; Key R&D Project in Agriculture and Animal Husbandrys of Rongchang, Chongqing[cstc2020ngzx0001] ; Fundamental Research Funds for the Central Universities[xtr042019020] ; Key R&D Program of Shaanxi Province[2020GXLH-Y016] ; Natural Science Foundation of Shaanxi Province[2020JM-055] ; Young Talents Support Plan of Xi'an Jiaotong University[HG6J002] |
| WOS研究方向 | Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000804211500006 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/16245]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Liu, Shuai; Li, Jianzhong; Zhou, Dezhong |
| 作者单位 | 1.Xi An Jiao Tong Univ, Sch Chem Engn & Technol, Xian 710049, Peoples R China 2.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China 3.Zhejiang Univ, Coll Pharmaceut Sci, Hangzhou 310058, Peoples R China 4.Xi An Jiao Tong Univ, Dept Thorac Surg, Affiliated Hosp 2, Xian 710061, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Qiuxia,Sun, Litao,Huang, Xiaobei,et al. Genetic Engineering of Adipose-Derived Stem Cells Using Biodegradable and Lipid-Like Highly Branched Poly(beta-amino ester)s[J]. ACS MACRO LETTERS,2022,11(5):636-642. |
| APA | Li, Qiuxia.,Sun, Litao.,Huang, Xiaobei.,Liu, Shuai.,Yong, Haiyang.,...&Zhou, Dezhong.(2022).Genetic Engineering of Adipose-Derived Stem Cells Using Biodegradable and Lipid-Like Highly Branched Poly(beta-amino ester)s.ACS MACRO LETTERS,11(5),636-642. |
| MLA | Li, Qiuxia,et al."Genetic Engineering of Adipose-Derived Stem Cells Using Biodegradable and Lipid-Like Highly Branched Poly(beta-amino ester)s".ACS MACRO LETTERS 11.5(2022):636-642. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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