Encapsulins from Ca. Brocadia fulgida: An effective tool to enhance the tolerance of engineered bacteria (pET-28a-cEnc) to Zn2+
文献类型：期刊论文
| 作者 | Wang, Que1; Zhou, Yue-Ming2 ; Xing, Chong-Yang3,4; Li, Wen-Chao1; Shen, Yu2; Yan, Peng1; Guo, Jin -Song1; Fang, Fang1; Chen, You -Peng1
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| 刊名 | JOURNAL OF HAZARDOUS MATERIALS
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| 出版日期 | 2022-08-05 |
| 卷号 | 435页码:11 |
| ISSN号 | 0304-3894 |
| 关键词 | Encapsulins Zn2+ Overexpression Escherichia coli Proteomics |
| DOI | 10.1016/j.jhazmat.2022.128954 |
| 通讯作者 | Chen, You -Peng(ypchen@cqu.edu.cn) |
| 英文摘要 | Zn2+ is largely discharged from many industries and poses a severe threat to the environment, making its remediation crucial. Encapsulins, proteinaceous nano-compartments, may protect cells against environmental stresses by sequestering toxic substances. To determine whether hemerythrin-containing encapsulins (cEnc) from anammox bacteria Ca. Brocadia fulgida can help cells deal with toxic substances such as Zn2+, we transferred cEnc into E.coli by molecular biology technologies for massive expression and then cultured them in media with increasing Zn2+ levels. The engineered bacteria (with cEnc) grew better and entered the apoptosis phase later, while wild bacteria showed poor survival. Furthermore, tandem mass tag-based quantitative proteomic analysis was used to reveal the underlying regulatory mechanism by which the genetically-engineered bacteria (with cEnc) adapted to Zn2+ stress. When Zn2+ was sequestered in cEnc as a transition, the engineered bacteria pre-sented a complex network of regulatory systems against Zn2+-induced cytotoxicity, including functions related to ribosomes, sulfur metabolism, flagellar assembly, DNA repair, protein synthesis, and Zn2+ efflux. Our findings offer an effective and promising stress control strategy to enhance the Zn2+ tolerance of bacteria for Zn2+ remediation and provide a new application for encapsulins. |
| 资助项目 | National Natural Science Foundation of China[21876016] ; National Natural Science Foundation of China[51878091] ; National Natural Science Foundation of China[52070031] ; Chongqing Talents: Exceptional Young Talents Project |
| WOS研究方向 | Engineering ; Environmental Sciences & Ecology |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000798745400005 |
| 源URL | [http://119.78.100.138/handle/2HOD01W0/16263]  |
| 专题 | 中国科学院重庆绿色智能技术研究院 |
| 通讯作者 | Chen, You -Peng |
| 作者单位 | 1.Chongqing Univ, Key Lab Three Gorges Reservoir Reg Ecoenvironm, MOE, Chongqing 400045, Peoples R China 2.Chongqing Technol & Business Univ, Natl Base Int Sci &Technol Cooperat Intelligent Mf, Chongqing 400067, Peoples R China 3.Chinese Acad Sci, Chongqing Inst Green & Intelligence Technol, Key Lab Reservoir Aquat Environm, Chongqing 400714, Peoples R China 4.Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400714, Peoples R China |
| 推荐引用方式 GB/T 7714 |
Wang, Que,Zhou, Yue-Ming,Xing, Chong-Yang,et al. Encapsulins from Ca. Brocadia fulgida: An effective tool to enhance the tolerance of engineered bacteria (pET-28a-cEnc) to Zn2+ [J]. JOURNAL OF HAZARDOUS MATERIALS,2022,435:11. |
| APA |
Wang, Que.,Zhou, Yue-Ming.,Xing, Chong-Yang.,Li, Wen-Chao.,Shen, Yu.,...&Chen, You -Peng.(2022). Encapsulins from Ca. Brocadia fulgida: An effective tool to enhance the tolerance of engineered bacteria (pET-28a-cEnc) to Zn2+ .JOURNAL OF HAZARDOUS MATERIALS,435,11. |
| MLA |
Wang, Que,et al." Encapsulins from Ca. Brocadia fulgida: An effective tool to enhance the tolerance of engineered bacteria (pET-28a-cEnc) to Zn2+ ".JOURNAL OF HAZARDOUS MATERIALS 435(2022):11. |
入库方式： OAI收割
来源：重庆绿色智能技术研究院
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