Analysis of Purified Okadaic Acid and Dinophysis Toxin 1 from Prorocentrum Lima Dodge
文献类型：期刊论文
| 作者 | Feng Zhen-Zhou1,2; Yu Ren-Cheng1 ; Zhou Ming-Jiang1
|
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2012-04-01 |
| 卷号 | 40期号:4页码:539-544 |
| 关键词 | Diarrhetic shellfish poisoning Okadaic acid Dinophysis toxin 1 High performance liquid chromatography-mass spectrometry |
| ISSN号 | 0253-3820 |
| 通讯作者 | Yu, RC (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China. |
| 英文摘要 | Okadaic acid (OA) and dinophysis toxins (DTXs) are representative toxins corresponding for the diarrhetic shellfish poisoning (DSP) incidents. In the current study, the samples of OA and DTX1, which were purified previously from the cultured algal cells of dinoflagellate Prorocentrum lima Dodge, were analyzed for the identity and purity with high performance liquid chromatography coupled with fluorescence detector (HPLC-FLD) and ultraviolet detector (HPLC-UV), mass spectrometry (MS) and HPLC-MS. Commercially available toxin standards of OA and DTX1 were also analyzed to compare with the purified samples. After derivatized with 4-bromomethyl-7-methoxycoumarin (BrMmc), OA and DTX1 were detected in the purified samples with the HPLC-FLD method. In the HPLC-UV chromatogrphy detected at 200 nm, OA and DTX1 were found to be the most predominant peaks, and the purity calculated based on the peak area could reach 95% and 98% for the samples of OA and DTX1, respectively. In the MS spectrums of the purified samples and toxin standards, the highest signals in the positive mode or the negative mode had the same value of m/z, which could be identified as [M+Na](+) and [M-H](-) of OA and DTX1. The [M+Na](+) and [M-H](-) ions of the toxin samples showed similar fragmentation pattern as those of the toxin standards, further confirming the presence of OA and DTX1 in the purified samples. In the LC-MS analysis of the purified samples with the full-scan m/z range at 100-1300, a small amount of impurities in the OA sample was found, and no impurity presented in the DTX1 sample. The results suggested that the OA and DTX1 samples prepared previously had high purity and could be used as standards for the analysis of OA and DTX1 with HPLC-FLD and HPLC-MS methods in management and protection of seafood. |
| 学科主题 | Chemistry, Analytical |
| 收录类别 | SCI |
| 原文出处 | 10.3724/SP.J.1096.2012.00741 |
| 语种 | 英语 |
| WOS记录号 | WOS:000313810800007 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12413]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng Zhen-Zhou,Yu Ren-Cheng,Zhou Ming-Jiang. Analysis of Purified Okadaic Acid and Dinophysis Toxin 1 from Prorocentrum Lima Dodge[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2012,40(4):539-544. |
| APA | Feng Zhen-Zhou,Yu Ren-Cheng,&Zhou Ming-Jiang.(2012).Analysis of Purified Okadaic Acid and Dinophysis Toxin 1 from Prorocentrum Lima Dodge.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,40(4),539-544. |
| MLA | Feng Zhen-Zhou,et al."Analysis of Purified Okadaic Acid and Dinophysis Toxin 1 from Prorocentrum Lima Dodge".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 40.4(2012):539-544. |
入库方式： OAI收割
来源：海洋研究所
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