Structural properties of the tubular appendage spinae from marine bacterium Roseobacter sp strain YSCB
文献类型：期刊论文
| 作者 | Bernadac, A.2; Wu, L. -F.1,8; Santini, C. -L.1,8; Vidaud, C.3; Sturgis, J. N.4; Menguy, N.5,8; Bergam, P.2; Nicoletti, C.6; Xiao, T.7,8 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2012-12-10 |
| 卷号 | 2页码:950 |
| 关键词 | DIFFERENTIAL SCANNING CALORIMETRY AMYLOID FIBRILS PROTEIN CHLOROBIUM COLONIZATION BIOGENESIS STABILITY LARVAE CLADE |
| ISSN号 | 2045-2322 |
| 通讯作者 | Wu, LF (reprint author), Aix Marseille Univ, Inst Microbiol Mediterranee, CNRS, Lab Chim Bacterienne,UMR 7283, 31 Chem Joseph Aiguier, F-13402 Marseille 20, France. |
| 英文摘要 | Spinae are tubular surface appendages broadly found in Gram-negative bacteria. Little is known about their architecture, function or origin. Here, we report structural characterization of the spinae from marine bacteria Roseobacter sp. YSCB. Electron cryo-tomography revealed that a single filament winds into a hollow flared base with progressive change to a cylinder. Proteinase K unwound the spinae into proteolysis-resistant filaments. Thermal treatment ripped the spinae into ribbons that were melted with prolonged heating. Circular dichroism spectroscopy revealed a dominant beta-structure of the spinae. Differential scanning calorimetry analyses showed three endothermic transformations at 50-85 degrees C, 98 degrees C and 123 degrees C, respectively. The heating almost completely disintegrated the spinae, abolished the 98 degrees C transition and destroyed the beta-structure. Infrared spectroscopy identified the amide I spectrum maximum at a position similar to that of amyloid fibrils. Therefore, the spinae distinguish from other bacterial appendages, e.g. flagella and stalks, in both the structure and mechanism of assembly. |
| 学科主题 | Multidisciplinary Sciences |
| 收录类别 | SCI |
| 原文出处 | 10.1038/srep00950 |
| 语种 | 英语 |
| WOS记录号 | WOS:000312273400009 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12442]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Aix Marseille Univ, Inst Microbiol Mediterranee, CNRS, Lab Chim Bacterienne,UMR 7283, F-13402 Marseille 20, France 2.Aix Marseille Univ, Inst Microbiol Mediterranee, CNRS, Serv Microscopie Elect, F-13402 Marseille 20, France 3.CEA Marcoule, Inst Biol Environm & Biotechnol, Lab Target Prot Studies, F-30207 Bagnolssur Ceze, France 4.Aix Marseille Univ, Inst Microbiol Mediterranee, CNRS, Lab Ingn Syst Macromol,UMR 7255, F-13402 Marseille 20, France 5.Univ Paris 06, IMPMC, UMR 7590, CNRS,IPGP,IRD, Paris 05, France 6.Aix Marseille Univ, CNRS, ISM2, UMR 7313, F-13397 Marseille 20, France 7.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 8.CNRS, Lab Int Assoc Biomineralisat & Nanostruct LIA Bio, F-13402 Marseille 20, France |
| 推荐引用方式 GB/T 7714 | Bernadac, A.,Wu, L. -F.,Santini, C. -L.,et al. Structural properties of the tubular appendage spinae from marine bacterium Roseobacter sp strain YSCB[J]. SCIENTIFIC REPORTS,2012,2:950. |
| APA | Bernadac, A..,Wu, L. -F..,Santini, C. -L..,Vidaud, C..,Sturgis, J. N..,...&Xiao, T..(2012).Structural properties of the tubular appendage spinae from marine bacterium Roseobacter sp strain YSCB.SCIENTIFIC REPORTS,2,950. |
| MLA | Bernadac, A.,et al."Structural properties of the tubular appendage spinae from marine bacterium Roseobacter sp strain YSCB".SCIENTIFIC REPORTS 2(2012):950. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。