Functionalized magnetic mesoporous silica nanoparticles: Fabrication, laccase adsorption performance and direct laccase capture from Trametes versicolor fermentation broth
文献类型：期刊论文
| 作者 | Wang, Feng1; Huang, Wei2; Guo, Chen1; Liu, Chun-Zhao1 |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2012-12-01 |
| 卷号 | 126期号:0页码:117-122 |
| 关键词 | Mesoporous Size-selective Purification Laccase Magnetic separation |
| ISSN号 | 0960-8524 |
| 通讯作者 | Liu, CZ |
| 英文摘要 | A simple and highly efficient protocol using magnetic mesoporous silica nanoparticles (MMSNPs) with metal affinity ligands was developed to directly capture laccase from Trametes versicolor fermentation broth. The Cu2+-chelated magnetic mesoporous silica nanoparticles (MMSNPs-Cu2+) with pore sizes ranging from 3.6 to 27.1 nm exhibited size selectivity on laccase capture from the fermentation broth, and the MMSNPs-Cu2+ with an average pore size of 14.5 nm provided 60.6-fold purification of laccase and 114.6% recovery yield of enzyme activity. Both size selectivity of the MMSNPs and affinity of the chelated metal ion resulted in high laccase capture efficiency from the fermentation broth. The most efficient MMSNPs-Cu2+ demonstrated no significant loss in laccase capture effectiveness following 10 reuse cycles. This simple and efficient strategy has the potential to be used for the robust and inexpensive preparation of purified laccase at the industrial scale. (C) 2012 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| 类目[WOS] | Agricultural Engineering ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 研究领域[WOS] | Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels |
| 关键词[WOS] | WORMHOLE FRAMEWORK STRUCTURES ; PROTEIN SEPARATION ; PURIFICATION ; IMMOBILIZATION ; MESOSTRUCTURE ; ENRICHMENT ; SPHERES ; SYSTEM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000313997500017 |
| 公开日期 | 2013-09-30 |
| 版本 | 出版稿 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/2687]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Beijing Res Inst Nutr Resources, Beijing 100069, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Feng,Huang, Wei,Guo, Chen,et al. Functionalized magnetic mesoporous silica nanoparticles: Fabrication, laccase adsorption performance and direct laccase capture from Trametes versicolor fermentation broth[J]. BIORESOURCE TECHNOLOGY,2012,126(0):117-122. |
| APA | Wang, Feng,Huang, Wei,Guo, Chen,&Liu, Chun-Zhao.(2012).Functionalized magnetic mesoporous silica nanoparticles: Fabrication, laccase adsorption performance and direct laccase capture from Trametes versicolor fermentation broth.BIORESOURCE TECHNOLOGY,126(0),117-122. |
| MLA | Wang, Feng,et al."Functionalized magnetic mesoporous silica nanoparticles: Fabrication, laccase adsorption performance and direct laccase capture from Trametes versicolor fermentation broth".BIORESOURCE TECHNOLOGY 126.0(2012):117-122. |
入库方式： OAI收割
来源：过程工程研究所
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