Stable, Bright, and Long-Fluorescence-Lifetime Dyes for Deep-Near- Infrared Bioimaging
文献类型：期刊论文
| 作者 | Li, Jin8,9; Dong, Yan8,9; Wei, Ruwei8,9; Jiang, Guanyu7; Yao, Cheng8,9; Lv, Meng7; Wu, Yuyang6; Gardner, Sarah H.1,5; Zhang, Feng4; Lucero, Melissa Y.1,5 |
| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| 出版日期 | 2022-07-29 |
| 页码 | 12 |
| ISSN号 | 0002-7863 |
| DOI | 10.1021/jacs.2c05826 |
| 通讯作者 | Yang, Youjun(youjunyang@ecust.edu.cn) |
| 英文摘要 | Near-infrared (NIR) fluorophores absorbing maximally in the region beyond 800 nm, i.e., deep-NIR spectral region, are actively sought for biomedical applications. Ideal dyes are bright, nontoxic, photostable, biocompatible, and easily derivatized to introduce functionalities (e.g., for bioconjugation or aqueous solubility). The rational design of such fluorophores remains a major challenge. Silicon-substituted rhodamines have been successful for bioimaging applications in the red spectral region. The longer wavelength silicon-substituted congeners for the deep-NIR spectral region are unknown to date. We successfully prepared four silicon substituted bis-benzannulated rhodamine dyes (ESi5a-ESi5d), with an efficient five-step cascade on a gram-scale. Because of the extensive overlapping of their HOMO-LUMO orbitals, ESi5a-ESi5d are highly absorbing (lambda abs & AP; 865 nm and epsilon > 105 cm-1 M-1). By restraining both the rotational freedom via annulation and the vibrational freedom via silicon-imparted strain, the fluorochromic scaffold of ESi5 is highly rigid, resulting in an unusually long fluorescence lifetime (tau > 700 ps in CH2Cl2) and a high fluorescence quantum yield (phi = 0.14 in CH2Cl2). Their half-lives toward photobleaching are 2 orders of magnitude longer than the current standard (ICG in serum). They are stable in the presence of biorelevant concentration of nucleophiles or reactive oxygen species. They are minimally toxic and readily metabolized. Upon tail vein injection of ESi5a (as an example), the vasculature of a nude mouse was imaged with a high signal-to-background ratio. ESi5 dyes have broad potentials for bioimaging in the deep-NIR spectral region. |
| WOS关键词 | IN-VIVO ; POLYMETHINE DYES ; ORGANIC FLUOROPHORES ; ABSORPTION-SPECTRA ; II FLUOROPHORES ; CYANINE DYE ; RHODAMINE ; NANOPARTICLES ; EMISSION ; DESIGN |
| 资助项目 | National Natural Science Foundation of China[21822805] ; National Natural Science Foundation of China[21908065] ; National Natural Science Foundation of China[12034008] ; National Natural Science Foundation of China[91850202] ; National Natural Science Foundation of China[21873030] ; National Natural Science Foundation of China[11674101] ; National Natural Science Foundation of China[81922070] ; National Natural Science Foundation of China[22078098] ; China Postdoctoral Science Foundation[2019M651427] ; China Postdoctoral Science Foundation[2020T130197] ; Shanghai Rising-Star Program[21QA1402600] ; Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission) ; Research Fund of Happiness Flower ECNU[2020JK2103] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000835523000001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/301989]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Yang, Youjun |
| 作者单位 | 1.Univ Illinois, Dept Chem, Urbana, IL 61801 USA 2.East China Normal Univ, Sch Chem & Mol Engn, Shanghai 200241, Peoples R China 3.Shanghai Inst Food & Drug Control, Pharmacol & Toxicol Div, Shanghai 201203, Peoples R China 4.Shanghai Univ Tradit Chinese Med, Inst Interdisciplinary Integrat Med Res, Shanghai Frontiers Sci Ctr Chinese Med Chem Biol, Shanghai 201203, Peoples R China 5.Univ Illinois, Beckman Inst Adv Sci & Technol, Urbana, IL 61801 USA 6.Chinese Acad Sci, Shanghai Inst Mat Med, Mol Imaging Ctr, Shanghai 201203, Peoples R China 7.East China Normal Univ, Sch Phys & Elect Sci, State Key Lab Precis Spect, Shanghai 200241, Peoples R China 8.East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China 9.East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jin,Dong, Yan,Wei, Ruwei,et al. Stable, Bright, and Long-Fluorescence-Lifetime Dyes for Deep-Near- Infrared Bioimaging[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2022:12. |
| APA | Li, Jin.,Dong, Yan.,Wei, Ruwei.,Jiang, Guanyu.,Yao, Cheng.,...&Yang, Youjun.(2022).Stable, Bright, and Long-Fluorescence-Lifetime Dyes for Deep-Near- Infrared Bioimaging.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,12. |
| MLA | Li, Jin,et al."Stable, Bright, and Long-Fluorescence-Lifetime Dyes for Deep-Near- Infrared Bioimaging".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2022):12. |
入库方式： OAI收割
来源：上海药物研究所
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