Divergence in Enzymatic Activities in the Soybean GST Supergene Family Provides New Insight into the Evolutionary Dynamics of Whole-Genome Duplicates
文献类型：期刊论文
| 作者 | Liu, Hai-Jing; Tang, Zhen-Xin; Han, Xue-Min; Yang, Zhi-Ling; Zhang, Fu-Min; Yang, Hai-Ling; Liu, Yan-Jing; Zeng, Qing-Yin |
| 刊名 | MOLECULAR BIOLOGY AND EVOLUTION
 |
| 出版日期 | 2015 |
| 卷号 | 32期号:11页码:2844-2859 |
| 关键词 | gene and genome duplication gene family glutathione S-transferase enzyme activity functional divergence |
| ISSN号 | 0737-4038 |
| DOI | 10.1093/molbev/msv156 |
| 文献子类 | Article |
| 英文摘要 | Whole-genome duplication (WGD), or polyploidy, is a major force in plant genome evolution. A duplicate of all genes is present in the genome immediately following a WGD event. However, the evolutionary mechanisms responsible for the loss of, or retention and subsequent functional divergence of polyploidy-derived duplicates remain largely unknown. In this study we reconstructed the evolutionary history of the glutathione S-transferase (GST) gene family from the soybean genome, and identified 72 GST duplicated gene pairs formed by a recent Glycine-specific WGD event occurring approximately 13 Ma. We found that 72% of duplicated GST gene pairs experienced gene losses or pseudogenization, whereas 28% of GST gene pairs have been retained in the soybean genome. The GST pseudogenes were under relaxed selective constraints, whereas functional GSTs were subject to strong purifying selection. Plant GST genes play important roles in stress tolerance and detoxification metabolism. By examining the gene expression responses to abiotic stresses and enzymatic properties of the ancestral and current proteins, we found that polyploidy-derived GST duplicates show the divergence in enzymatic activities. Through site-directed mutagenesis of ancestral proteins, this study revealed that nonsynonymous substitutions of key amino acid sites play an important role in the divergence of enzymatic functions of polyploidy-derived GST duplicates. These findings provide new insights into the evolutionary and functional dynamics of polyploidy-derived duplicate genes. |
| 学科主题 | Biochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1537-1719 |
| WOS关键词 | FUNCTIONAL DIVERGENCE ; GLUTATHIONE TRANSFERASES ; GENE FAMILY ; POLYPLOIDY ; CONSEQUENCES ; ACID ; TRIPLICATION ; EXPRESSION ; SEQUENCE ; EVENTS |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000363033100004 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | National Science Foundation of China [91231103, 31425006] ; Chinese Academy of Sciences [KSCX2-EW-J-1] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25740]  |
| 专题 | 植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文 |
| 作者单位 | 1.Yang, Zhi-Ling 2.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing, Peoples R China 3.Han, Xue-Min 4.[Liu, Hai-Jing 5.Zhang, Fu-Min 6.Liu, Yan-Jing 7.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China 8.Univ Chinese Acad Sci, Beijing, Peoples R China 9.[Tang, Zhen-Xin |
| 推荐引用方式 GB/T 7714 | Liu, Hai-Jing,Tang, Zhen-Xin,Han, Xue-Min,et al. Divergence in Enzymatic Activities in the Soybean GST Supergene Family Provides New Insight into the Evolutionary Dynamics of Whole-Genome Duplicates[J]. MOLECULAR BIOLOGY AND EVOLUTION,2015,32(11):2844-2859. |
| APA | Liu, Hai-Jing.,Tang, Zhen-Xin.,Han, Xue-Min.,Yang, Zhi-Ling.,Zhang, Fu-Min.,...&Zeng, Qing-Yin.(2015).Divergence in Enzymatic Activities in the Soybean GST Supergene Family Provides New Insight into the Evolutionary Dynamics of Whole-Genome Duplicates.MOLECULAR BIOLOGY AND EVOLUTION,32(11),2844-2859. |
| MLA | Liu, Hai-Jing,et al."Divergence in Enzymatic Activities in the Soybean GST Supergene Family Provides New Insight into the Evolutionary Dynamics of Whole-Genome Duplicates".MOLECULAR BIOLOGY AND EVOLUTION 32.11(2015):2844-2859. |
入库方式： OAI收割
来源：植物研究所
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