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Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation

文献类型：期刊论文

作者Zhu, Zhixin2; Wang, Hailong2; Wang, Yiting2; Guan, Shan; Wang, Fang2; Tang, Jingyu2; Zhang, Ruijuan2; Xie, Lulu2; Lu, Yingqing2
刊名JOURNAL OF EXPERIMENTAL BOTANY
出版日期2015
卷号66期号:13页码:3775-3789
关键词Anthocyanin pathway Arabidopsis bHLH cis element Ipomoea MBW complex MYB promoter activity promoter architecture regulatory module trans factor transcription initiation WDR
ISSN号0022-0957
DOI10.1093/jxb/erv173
文献子类Article
英文摘要Cellular activities such as compound synthesis often require the transcriptional activation of an entire pathway; however, the molecular mechanisms underlying pathway activation have rarely been explained. Here, the cis regulatory architecture of the anthocyanin pathway genes targeted by the transcription factor (TF) complex including MYB, bHLH, and WDR was systematically analysed in one species and the findings extended to others. In Ipomoea purpurea, the IpMYB1-IpbHLH2-IpWDR1 (IpMBW) complex was found to be orthologous to the PAP1-GL3-TTG1 (AtPGT) complex of Arabidopsis thaliana, and interacted with a 7-bp MYB-recognizing element (MRE) and a 6-bp bHLH-recognizing element (BRE) at the proximal promoter region of the pathway genes. There was little transcription of the gene in the absence of the MRE or BRE. The cis elements identified experimentally converged on two syntaxes, ANCNNCC for MREs and CACN(A/C/T)(G/T) for BREs, and our bioinformatic analysis showed that these were present within anthocyanin gene promoters in at least 35 species, including both gymnosperms and angiosperms. For the anthocyanin pathway, IpMBW and AtPGT recognized the interspecific promoters of both early and later genes. In A. thaliana, the seed-specific TF complex (TT2, TT8, and TTG1) may regulate all the anthocyanin pathway genes, in addition to the proanthocyanidin-specific BAN. When multiple TF complexes in the anthocyanin pathway were compared, the cis architecture played a role larger than the TF complex in determining the variation in promoter activity. Collectively, a cis logic common to the pathway gene promoters was found, and this logic is essential for the trans factors to regulate the pathway.
学科主题Plant Sciences
出版地OXFORD
电子版国际标准刊号1460-2431
WOS关键词CHALCONE SYNTHASE PROMOTER ; ARABIDOPSIS-THALIANA ; MORNING GLORY ; TRANSCRIPTION FACTORS ; REPEAT PROTEIN ; MYB DOMAIN ; PHENYLPROPANOID BIOSYNTHESIS ; EXPRESSION PATTERNS ; FLAVONOID SYNTHESIS ; IPOMOEA-PURPUREA
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000358533400010
出版者OXFORD UNIV PRESS
资助机构National Science Foundation of China [91331116, 31070263] ; Chinese Academy of Sciences [KSCX2-YW-N-043]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/25790]  
专题植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714		 Zhu, Zhixin,Wang, Hailong,Wang, Yiting,et al. Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation[J]. JOURNAL OF EXPERIMENTAL BOTANY,2015,66(13):3775-3789.
APA Zhu, Zhixin.,Wang, Hailong.,Wang, Yiting.,Guan, Shan.,Wang, Fang.,...&Lu, Yingqing.(2015).Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation.JOURNAL OF EXPERIMENTAL BOTANY,66(13),3775-3789.
MLA Zhu, Zhixin,et al."Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation".JOURNAL OF EXPERIMENTAL BOTANY 66.13(2015):3775-3789.

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来源：植物研究所

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