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Isolation and characterization of 18 polymorphic microsatellite markers for the Female Ginseng Angelica sinensis (Apiaceae) and cross-species amplification

文献类型：期刊论文

作者Lu, Youyuan3; Cheng, Tao2; Zhu, Tiantian; Jiang, Dan3; Zhou, Shiliang2; Jin, Ling; Yuan, Qingjun3; Huang, Luqi3
刊名BIOCHEMICAL SYSTEMATICS AND ECOLOGY
出版日期2015
卷号61页码:488-492
关键词Angelica sinensis Medicinal plant Microsatellite Molecular marker Cross-amplification Population genetics
ISSN号0305-1978
DOI10.1016/j.bse.2015.07.013
文献子类Article
英文摘要Angelica sinensis (Apiaceae) is an endangered alpine herb that is widely used as a medicinal plant in traditional Chinese medicine (TCM). Wild populations of A. sinensis have become quite rare in China. Thus, population genetics studies of this species are urgently needed for its effective conservation and sustainable use. However, to date, no microsatellite loci have been isolated in A. sinensis. To address this issue, we isolated 18 polymorphic loci and genotyped 120 individuals collected from 6 populations. The number of alleles per locus ranged from 1.2 to 5.5, and the average was 2.4. The observed and expected heterozygosity per locus for a population varied, respectively, from 0.000 to 0.983 (averaged at 0.198) and from 0.066 to 0.661 (averaged at 0.333). Deviation from the Hardy-Weinberg equilibrium (p < 0.01) was observed for 4 to 14 loci in various populations. These microsatellite markers were cross-amplified in 10 species affinis, and 7 loci were successfully amplified in all species. These microsatellite markers are useful for genetic studies, the conservation management of A. sinensis, and identification of A. sinensis. (C) 2015 Elsevier Ltd. All rights reserved.
学科主题Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
出版地OXFORD
电子版国际标准刊号1873-2925
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000361164800068
出版者PERGAMON-ELSEVIER SCIENCE LTD
资助机构National Natural Science Foundation of China (NSFC) [81360615, 81274027, 81130070] ; Beijing Joint Project Specific Funds ; Independent Project of Basic Scientific Research Operating Expenses of Institute of Chinese Materia Medial, China Academy of Chinese Medical Sciences [2011ZDXK-01]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/25821]  
专题植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文
作者单位1.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China
2.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Breeding Base Daodi Herbs, Beijing 100700, Peoples R China
3.Gansu Univ Chinese Med, Lanzhou 730000, Gansu, Peoples R China
推荐引用方式
GB/T 7714		 Lu, Youyuan,Cheng, Tao,Zhu, Tiantian,et al. Isolation and characterization of 18 polymorphic microsatellite markers for the Female Ginseng Angelica sinensis (Apiaceae) and cross-species amplification[J]. BIOCHEMICAL SYSTEMATICS AND ECOLOGY,2015,61:488-492.
APA Lu, Youyuan.,Cheng, Tao.,Zhu, Tiantian.,Jiang, Dan.,Zhou, Shiliang.,...&Huang, Luqi.(2015).Isolation and characterization of 18 polymorphic microsatellite markers for the Female Ginseng Angelica sinensis (Apiaceae) and cross-species amplification.BIOCHEMICAL SYSTEMATICS AND ECOLOGY,61,488-492.
MLA Lu, Youyuan,et al."Isolation and characterization of 18 polymorphic microsatellite markers for the Female Ginseng Angelica sinensis (Apiaceae) and cross-species amplification".BIOCHEMICAL SYSTEMATICS AND ECOLOGY 61(2015):488-492.

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来源：植物研究所

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