Methylation mediated by an anthocyanin, O-methyltransferase, is involved in purple flower coloration in Paeonia
文献类型：期刊论文
| 作者 | Du, Hui; Wu, Jie4; Ji, Kui-Xian; Zeng, Qing-Yin; Bhuiya, Mohammad-Wadud; Su, Shang4; Shu, Qing-Yan; Ren, Hong-Xu; Liu, Zheng-An ; Wang, Liang-Sheng
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| 刊名 | JOURNAL OF EXPERIMENTAL BOTANY
 |
| 出版日期 | 2015 |
| 卷号 | 66期号:21页码:6563-6577 |
| 关键词 | Anthocyanin O-methyltransferase catalytic activity flavonoids flower coloration Paeonia single amino acid substitution |
| ISSN号 | 0022-0957 |
| DOI | 10.1093/jxb/erv365 |
| 文献子类 | Article |
| 英文摘要 | Anthocyanins are major pigments in plants. Methylation plays a role in the diversity and stability of anthocyanins. However, the contribution of anthocyanin methylation to flower coloration is still unclear. We identified two homologous anthocyanin O-methyltransferase (AOMT) genes from purple-flowered (PsAOMT) and red-flowered (PtAOMT) Paeonia plants, and we performed functional analyses of the two genes in vitro and in vivo. The critical amino acids for AOMT catalytic activity were studied by site-directed mutagenesis. We showed that the recombinant proteins, PsAOMT and PtAOMT, had identical substrate preferences towards anthocyanins. The methylation activity of PsAOMT was 60 times higher than that of PtAOMT in vitro. Interestingly, this vast difference in catalytic activity appeared to result from a single amino acid residue substitution at position 87 (arginine to leucine). There were significant differences between the 35S::PsAOMT transgenic tobacco and control flowers in relation to their chromatic parameters, which further confirmed the function of PsAOMT in vivo. The expression levels of the two homologous AOMT genes were consistent with anthocyanin accumulation in petals. We conclude that AOMTs are responsible for the methylation of cyanidin glycosides in Paeonia plants and play an important role in purple coloration in Paeonia spp. |
| 学科主题 | Plant Sciences |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1460-2431 |
| WOS关键词 | PETAL ANTHOCYANINS ; PROTEIN-STRUCTURE ; GENETIC-CONTROL ; CYCLAMEN SPP. ; COPIGMENTATION ; EVOLUTION ; BIOSYNTHESIS ; PIGMENT ; TOOL ; IDENTIFICATION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000367814500004 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | National Natural Science Foundation of China [30771521, 31071824] ; National High Technology Research and Development Program of China (863 program) [2011AA10020702] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/25868]  |
| 专题 | 植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 3.MOgene Green Chem, St Louis, MO 63132 USA 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing Bot Garden, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Hui,Wu, Jie,Ji, Kui-Xian,et al. Methylation mediated by an anthocyanin, O-methyltransferase, is involved in purple flower coloration in Paeonia[J]. JOURNAL OF EXPERIMENTAL BOTANY,2015,66(21):6563-6577. |
| APA | Du, Hui.,Wu, Jie.,Ji, Kui-Xian.,Zeng, Qing-Yin.,Bhuiya, Mohammad-Wadud.,...&Wang, Liang-Sheng.(2015).Methylation mediated by an anthocyanin, O-methyltransferase, is involved in purple flower coloration in Paeonia.JOURNAL OF EXPERIMENTAL BOTANY,66(21),6563-6577. |
| MLA | Du, Hui,et al."Methylation mediated by an anthocyanin, O-methyltransferase, is involved in purple flower coloration in Paeonia".JOURNAL OF EXPERIMENTAL BOTANY 66.21(2015):6563-6577. |
入库方式： OAI收割
来源：植物研究所
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